αSMA Osteoprogenitor Cells Contribute to the Increase in Osteoblast Numbers in Response to Mechanical Loading
| Autor: | Ivo Kalajzic, Natalie K.Y. Wee, V. N. Widjaja, J. S. Price, S. H. Windahl, Brya G. Matthews |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Mouse Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Mice Transgenic Lineage tracing Article Weight-Bearing 03 medical and health sciences Mice 0302 clinical medicine Endocrinology In vivo Osteogenesis Periosteum medicine Animals Orthopedics and Sports Medicine Tibia Progenitor cell Osteoprogenitor Muscle actin Cell Proliferation Osteoblasts Chemistry Stem Cells Osteoblast Cell Differentiation Actins Cell biology Axial loading Mice Inbred C57BL medicine.anatomical_structure Cortical bone 030101 anatomy & morphology Stress Mechanical Periosteal bone formation |
| Zdroj: | Calcified tissue international Matthews, B G, Wee, N K Y, Widjaja, V N, Price, J S, Kalajzic, I & Windahl, S H 2020, ' αSMA Osteoprogenitor Cells Contribute to the Increase in Osteoblast Numbers in Response to Mechanical Loading ', Calcified Tissue International, vol. 106, no. 2, pp. 208-217 . https://doi.org/10.1007/s00223-019-00624-y |
| ISSN: | 1432-0827 0171-967X |
| DOI: | 10.1007/s00223-019-00624-y |
| Popis: | Bone is a dynamic tissue that site-specifically adapts to the load that it experiences. In response to increasing load, the cortical bone area is increased, mainly through enhanced periosteal bone formation. This increase in area is associated with an increase in the number of bone-forming osteoblasts; however, the origin of the cells involved remains unclear. Alpha-smooth muscle actin (αSMA) is a marker of early osteoprogenitor cells in the periosteum, and we hypothesized that the new osteoblasts that are activated by loading could originate from αSMA-expressing cells. Therefore, we used an in vivo fate-mapping approach in an established axial loading model to investigate the role of αSMA-expressing cells in the load-induced increase in osteoblasts. Histomorphometric analysis was applied to measure the number of cells of different origin on the periosteal surface in the most load-responsive region of the mouse tibia. A single loading session failed to increase the number of periosteal αSMA-expressing cells and osteoblasts. However, in response to multiple episodes of loading, the caudal, but not the cranial, periosteal surface was lined with an increased number of osteoblasts originating from αSMA-expressing cells 5 days after the initial loading session. The proportion of osteoblasts derived from αSMA-labeled progenitors increased by 70% (p |
| Databáze: | OpenAIRE |
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