Genetic structure of a germplasm for hybrid breeding in rye (Secale cereale L.)

Autor: Jihad Orabi, Ahmed Jahoor, Peter Skov Kristensen, Nikolaj Meisner Vendelbo, Pernille Sarup
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Germplasm
Genotyping Techniques
Single Nucleotide Polymorphisms
Population genetics
Plant Science
01 natural sciences
Linkage Disequilibrium
Effective population size
Phylogeny
education.field_of_study
Multidisciplinary
Ecology
Plant Anatomy
Secale
Eukaryota
Agriculture
Gene Pool
Genomics
Plants
Genetic structure
Seeds
Medicine
Gene pool
Research Article
Genetic Markers
Ecological Metrics
Genetics and Breeding
Science
Population
Biology
Polymorphism
Single Nucleotide
03 medical and health sciences
Rye
Genetic variation
Genetics
Grasses
education
Evolutionary Biology
Population Biology
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Species Diversity
biology.organism_classification
Agronomy
Plant Breeding
030104 developmental biology
Evolutionary biology
Hybridization
Genetic
Population Genetics
010606 plant biology & botany
Zdroj: PLoS ONE, Vol 15, Iss 10, p e0239541 (2020)
PLoS ONE
Vendelbo, N M, Sarup, P, Orabi, J, Kristensen, P S & Jahoor, A 2020, ' Genetic structure of a germplasm for hybrid breeding in rye ( Secale cereale L.) ', PLOS ONE, vol. 15, no. 10, e0239541 . https://doi.org/10.1371/journal.pone.0239541
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0239541
Popis: Rye (Secale cereale L.) responds strongly to changes in heterozygosity with hybrids portraying strong heterosis effect on all developmental and yielding characteristics. In order to achieve the highest potential heterosis effect parental lines must originate from genetically distinct gene pools. Here we report the first comprehensive SNP-based population study of an elite germplasm using fertilization control system for hybrid breeding in rye that is genetically different to the predominating P-type. In total 376 inbred lines from Nordic Seed Germany GmbH were genotyped for 4419 polymorphic SNPs. The aim of this study was to confirm and quantify the genetic separation of parental populations, unveil their genetic characteristics and investigate underlying population structures. Through a palette of complimenting analysis, we confirmed a strong genetic differentiation (FST = 0.332) of parental populations validating the germplasms suitability for hybrid breeding. These were, furthermore, found to diverge considerably in several features with the maternal population portraying a strong population structure characterized by a narrow genetic profile, small effective population size and high genome-wise linkage disequilibrium. We propose that the employed male-sterility system putatively constitutes a population determining parameter by influencing the rate of introducing novel genetic variation to the parental populations. Functional analysis of linkage blocks led to identification of a conserved segment on the distal 4RL chromosomal region annotated to the Rfp3 male-fertility restoration 'Pampa' type gene. Findings of our study emphasized the immediate value of comprehensive population studies on elite breeding germplasms as a pre-requisite for application of genomic-based breeding techniques, introgression of novel material and to support breeder decisionmaking.
Databáze: OpenAIRE
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