Development of an animal model to study meshes used in genital prolapse surgery
| Autor: | A. Defossez, R.J. Courcol, L. Boulanger, Malik Boukerrou, Michel Cosson, Patrick Devos, C. Rubod, Annie Fruchart |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Microbiological culture Tissue integration Connective tissue Animal model Staphylococcus epidermidis Uterine Prolapse Medicine Animals Surgical Wound Infection Sex organ Rats Wistar Escherichia coli Infections biology business.industry Abdominal skin Prolapse surgery Obstetrics and Gynecology Staphylococcal Infections Surgical Mesh biology.organism_classification Urogenital Surgical Procedures Surgery Rats Disease Models Animal medicine.anatomical_structure Reproductive Medicine Female business Cystocele Biomedical engineering |
| Zdroj: | European journal of obstetrics, gynecology, and reproductive biology. 136(2) |
| ISSN: | 0301-2115 |
| Popis: | Objective The objective was to develop an animal model using bacterial inoculation to evaluate tissue integration and tolerance to meshes used in genital prolapse surgery. Study design We placed three different meshes under the abdominal skin of 120 Wistar rats: a polypropylene monofilament non-coated mesh (Parietene ® ), a polypropylene monofilament collagen-coated mesh (Ugytex ® ) and a polyethylene terephthalate mesh (Mersuture ® ). We performed bacterial inoculation just after implantation with 1 ml of 10 7 colonies forming unit (CFU) of Staphylococcus epidermidis or Escherichia coli . Rats were sacrificed 7, 14, 60, and 90 days after intervention. We used polarised light microscopy to analyse the collagen deposition and organisation. We quantified the inflammation cells. Bacterial analysis and quantification of the explanted meshes were performed. The exact Fisher's test and Kruskal–Wallis test were used for statistics. Results We did not find any significant difference between inoculated or non-inoculated meshes in terms of collagen deposition. The scarring process seemed stable at day 90. Tissue integration was best with the polypropylene meshes, which allowed the development of a well-organised, mature connective tissue. Inflammatory reaction was higher in inoculated meshes, but only at day 7. At day 90, we found a high number of macrophages and multinuclear cells around all the meshes. There was no significant difference between prostheses that had been inoculated and those that had not with regard to positive bacterial culture. Quantification of bacterial colonies decreased with time. Conclusion In this animal model, we did not find any clinically related difference in infection and tissue integration between the meshes used in genital prolapse. Such experimental studies must be carried out whenever new prostheses become available before their use is validated in common practice. |
| Databáze: | OpenAIRE |
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