Repeated Measurements of 11β-HSD-1 Activity in Subcutaneous Adipose Tissue from Lean, Abdominally Obese, and Type 2 Diabetes Subjects – No Change Following a Mixed Meal
| Autor: | P-A Jansson, L. Sjögren, T Persson, J de Schoolmeester, Mikaela Sjöstrand, A Rees, Jan W. Eriksson, D Gill, Jenny Palming |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Clinical Biochemistry Subcutaneous Fat Type 2 diabetes Mixed meal Biochemistry Body Mass Index Endocrinology Thinness Internal medicine 11-beta-Hydroxysteroid Dehydrogenase Type 1 Abdomen medicine Humans Insulin Obesity Fasting state Aged Anthropometry business.industry Biochemistry (medical) 11β hydroxysteroid dehydrogenase Reproducibility of Results Fasting Feeding Behavior General Medicine Middle Aged medicine.disease Glucose Diabetes Mellitus Type 2 Female Subcutaneous adipose tissue business Ex vivo |
| Zdroj: | Hormone and Metabolic Research. 42:798-802 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/s-0030-1254134 |
| Popis: | The aim of this study was to measure 11β-HSD-1 activity in subcutaneous adipose tissue by an ex vivo method in three subgroups; lean, obese, and type 2 diabetes subjects, both in the fasting state and after a mixed meal and to determine the variability and reproducibility of this method. Eighteen subjects were investigated; 6 lean, 6 abdominally obese, and 6 type 2 diabetes subjects (BMI 22 ± 1, 30 ± 3 and 31 ± 3 kg/m², respectively). Needle biopsies were taken repeatedly and an index of 11β-HSD-1 activity was measured as percent conversion of (3)H-cortisone to (3)H-cortisol/100 mg tissue. For two separate biopsies taken in the fasting state on the same day, the within subjects CV was 16% and the between CV was 36% for 11β-HSD-1 activity for all subjects. For two biopsies taken in the fasting state at two different days, the total within subjects CV was 38% and the between subjects CV was 46%. Lean subjects had lower 11β-HSD-1 activity (4.8 ± 1.5% conversion of ³H-cortisone to ³H-cortisol/100 mg tissue) than both obese (14.4 ± 1.6% conversion, p0.01) and type 2 diabetes subjects (11.7 ± 1.9% conversion, p0.05) in the fasting state. There was no effect of a meal on 11β-HSD-1 activity in any of the three groups. The conclusions from this study are: 1) the variation coefficient for the ex vivo adipose tissue 11β-HSD-1 activity method was ∼25% for repeat measures within subjects; 2) food intake had no major impact on enzyme activity; and 3) 11β-HSD-1 activity in subcutaneous adipose tissue was significantly increased in obese subjects with or without T2DM compared to lean subjects without diabetes. |
| Databáze: | OpenAIRE |
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