The tolZ gene of Escherichia coli is identified as the ftsH gene
Autor: | Jian Ning Qu, Koreaki Ito, Yoshinori Koyama, Shin-ichi Makino, Hiroshi Matsuzawa, Yoshinori Akiyama, Toshifumi Tomoyasu, Hiroyuki Adachi, Thru Ogura |
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Rok vydání: | 1996 |
Předmět: |
DNA
Bacterial Mutant Colicins Biology medicine.disease_cause Microbiology Plasmid ATP-Dependent Proteases Bacterial Proteins Escherichia coli medicine Cloning Molecular Molecular Biology Gene Suppressor mutation Genetics Mutation Escherichia coli Proteins Genetic Complementation Test Chromosome Mapping Membrane Proteins Molecular biology Open reading frame Genes Bacterial Colicin Research Article |
Zdroj: | Journal of Bacteriology. 178:3457-3461 |
ISSN: | 1098-5530 0021-9193 |
Popis: | Escherichia coli tolZ mutants are tolerant to colicins E2, E3, D, Ia, and Ib (Tol-), can grow on glucose but not on succinate or other nonfermentable carbon sources (Nfc-), and show temperature-sensitive growth (Ts). A 1.8-kb DNA fragment that complemented the tolZ mutation was cloned. The DNA fragment was sequenced, and one open reading frame was found. This frame was identical to a part of the E. coli FtsH protein, an ATP-dependent metalloprotease that binds to the cytoplasmic membrane. The tolZ gene was located at 69 min on the E. coli genetic map, and the mutation was complemented by a plasmid carrying the ftsH gene, indicating that the tolZ gene is identical to the ftsH gene. The mutated tolZ21 gene was also cloned and sequenced and was found to have a single base change that caused an amino acid alteration of His-418 to Tyr in the FtsH protein. The tolZ21 mutant showed Hfl- (high frequency of lysogenization) and Std- (stop transfer-defective) pheno-types, both of which are due to a mutation in the ftsH (hflB) gene. However, the ftsH1, ftsH101, and hflB29 mutants did not show Tol- and Nfc phenotypes. The tolZ21 mutant was found to have a suppressor mutation, named sfhC, which allowed cells to survive. The sfhC mutation alone caused no Tol-, Nfc-, Ts, or Hfl- phenotypes in the tolZ21 mutant. |
Databáze: | OpenAIRE |
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