Role of Endosomal Cathepsins in Entry Mediated by the Ebola Virus Glycoprotein
| Autor: | Amy H. Bouton, Judith M. White, Shutoku Matsuyama, Sue E. Delos, Kirsten Kabsch, Kathryn L. Schornberg |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Small interfering RNA
Cathepsin L viruses Immunology Filoviridae Endosomes medicine.disease_cause Microbiology Vesicular stomatitis Indiana virus Virus Cathepsin B Cricetinae Virology Chlorocebus aethiops medicine Animals RNA Small Interfering Mononegavirales Vero Cells Cathepsin Ebola virus biology Ebolavirus biology.organism_classification Cathepsins Virus-Cell Interactions Cysteine Endopeptidases Insect Science biology.protein Viral Fusion Proteins |
| Zdroj: | Journal of Virology |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.80.8.4174-4178.2006 |
| Popis: | Using chemical inhibitors and small interfering RNA (siRNA), we have confirmed roles for cathepsin B (CatB) and cathepsin L (CatL) in Ebola virus glycoprotein (GP)-mediated infection. Treatment of Ebola virus GP pseudovirions with CatB and CatL converts GP1 from a 130-kDa to a 19-kDa species. Virus with 19-kDa GP1 displays significantly enhanced infection and is largely resistant to the effects of the CatB inhibitor and siRNA, but it still requires a low-pH-dependent endosomal/lysosomal function. These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|