Isolation and Properties of a Ribonuclease-Deficient Mutant of Salmonella typhimurium
| Autor: | C. Timothy Wehr |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Salmonella typhimurium
Salmonella Mutant Genetics and Molecular Biology medicine.disease_cause Microbiology Ribonucleases Transduction Genetic medicine Ribonuclease Molecular Biology Gene Edetic Acid Nitrosoguanidines Recombination Genetic chemistry.chemical_classification Mutation biology Chromosome Mapping RNA Molecular biology Stimulation Chemical Enzyme assay Culture Media Cryptococcus RNA Bacterial Enzyme Genes chemistry RNA Ribosomal biology.protein |
| Zdroj: | Journal of Bacteriology. 114:96-102 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | A mutant of Salmonella typhimurium has been isolated that has less than 5% of the ribonuclease activity of the parent strain. Mutant screening and enzyme assays were done in the presence of ethylenediaminetetraacetic acid, a substance that activates ribonuclease I and inhibits other known microbial nucleases. Genetic mapping indicates that the mutation is located between the purE and gal genes on the Salmonella chromosome. A ribonuclease-deficient mutant that carries a deletion in the pyrF gene is unable to utilize ribonucleic acid as a pyrimidine source, whereas the pyrF parent with normal ribonuclease activity will grow. This suggests that the enzyme may perform a scavenge function in the utilization of exogenous ribonucleic acid. Loss of this enzyme seems to have no detrimental effects on the growth of Salmonella . |
| Databáze: | OpenAIRE |
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