Molecular characterization of potato virus YN isolates by PCR-RFLP. Differentiation of PVYN isolates by PCR-RFLP
| Autor: | V. Marie Jeanne Tordo, Laurent Glais, Suzanne Astier-Manifacier, C. Robaglia, C. Kerlan, M. Tribodet |
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| Přispěvatelé: | Station de pathologie végétale (BiO3P), Institut National de la Recherche Agronomique (INRA), Laboratoire de biologie cellulaire et moléculaire |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Genetics
Potyviridae [SDV]Life Sciences [q-bio] Potyvirus POLYMORPHISME DE RESTRICTION Plant Science Horticulture Biology biology.organism_classification Molecular biology Genome law.invention Potato virus Y law Genotype Typing Restriction fragment length polymorphism VIRUS Y POMME DE TERRE Agronomy and Crop Science Polymerase chain reaction ComputingMilieux_MISCELLANEOUS |
| Zdroj: | European Journal of Plant Pathology European Journal of Plant Pathology, Springer Verlag, 1996, 102, pp.655-662 |
| ISSN: | 0929-1873 1573-8469 |
| Popis: | Based on the sequence polymorphism in the 5′ terminal part of the viral genome, a range of PVYN isolates were characterized by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Three pairs of primers selected in the 5′ non-translated and P1 protein region were tested. Two of them yielded PCR products of about 1Kb from all isolates tested. Restriction analysis of the PCR products gave two distinct electrophoretic patterns, whichever of the three enzymes was used. In this way, the 18 isolates were separated into two easily identifiable subgroups. All tuber necrosing isolates (PVYNTN) were clustered in the same subgroup. |
| Databáze: | OpenAIRE |
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