Genetic variations of CYP2R1 (rs10741657) in Bangladeshi adults with low serum 25(OH)D level-A pilot study

Autor: Farzana Yeasmin Mukta, Kazi Lutfar Rahman, Ridwana Rahman, Qazi Shamima Akhter, Md. Sayedur Rahman, Samina Rahman, Sudipta Sarker
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Heredity
Physiology
Organic chemistry
Artificial Gene Amplification and Extension
Pilot Projects
Genetic analysis
Polymerase Chain Reaction
Biochemistry
Geographical Locations
Gene Frequency
Nucleic Acids
Genotype
Vitamin D
Bangladesh
Multidisciplinary
Vitamins
Physical sciences
Exact test
Chemistry
Genetic Mapping
Research Design
Creatinine
Population study
Cholestanetriol 26-Monooxygenase
Medicine
Female
Analysis of variance
Research Article
Adult
Asia
Science
Variant Genotypes
Biology
Research and Analysis Methods
Chemical compounds
Asian People
Genetic variation
Organic compounds
Genetics
Humans
Genetic Testing
Allele
Molecular Biology Techniques
Cytochrome P450 Family 2
Molecular Biology
Alleles
Chi-Square Distribution
Biology and Life Sciences
Genetic Variation
Pilot Studies
DNA
Minor allele frequency
People and Places
Biomarkers
Zdroj: PLoS ONE, Vol 16, Iss 11, p e0260298 (2021)
PLoS ONE
PLoS ONE, Vol 16, Iss 11 (2021)
ISSN: 1932-6203
Popis: Background Some studies revealed that despite having sufficient sun exposure and dietary supply, the level of serum 25(OH)D in Bangladeshi adults is lower than its normal range. Genetic pattern of an individual is also an essential factor that regulates the level of serum 25(OH)D. However, the genetic variations of CYP2R1 (rs10741657) and their association with low serum 25(OH)D level in Bangladeshi adults are yet to be explored. Objective This study was conducted to determine the frequency of variants of rs10741657 of CYP2R1 gene and its association with low serum 25(OH)D level among Bangladeshi adults. Method This pilot study was conducted among thirty individuals with low serum 25(OH)D level as the study population and ten subjects with sufficient serum 25(OH)D level as controls based on the inclusion and exclusion criteria. Genetic analysis of rs10741657 of CYP2R1 including primer designing, DNA extraction, PCR of target region with purification and Sanger sequencing of the PCR products were done accordingly. For statistical analysis, One-way ANOVA followed by LSD test, Freeman-Halton extension of Fisher’s exact test, Chi-square test (χ2) test and unpaired student t-test were performed. Results In this study, genetic variants of CYP2R1 (rs10741657) among the study population were genotype GG (63.30%), GA (30%) and AA (6.7%). Minor allele frequency of the study population was 0.217. The association between GG and GA genotypes of CYP2R1 (rs10741657) with low serum 25(OH)D level among the study population was found and it was statistically significant. Statistically significant differences were also observed between the genotypes and alleles of the study population and controls. Conclusions The presence of ‘GG’ and ‘GA’ genotypes of rs1041657 in CYP2R1 gene is associated with low serum 25(OH)D level among Bangladeshi adults in this pilot study.
Databáze: OpenAIRE
Externí odkaz: