Isolation and characterization of a Rhodococcus strain able to degrade 2-fluorophenol
| Autor: | Maria F. Carvalho, Ghufrana Samin, Vânia S. Bessa, Dick B. Janssen, Syed A. Hasan, Paula M. L. Castro, Anouk F. Duque |
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| Přispěvatelé: | Biotechnology, Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
OXIDOREDUCTASE TFTC
BIODEGRADATION Applied Microbiology and Biotechnology RNA Ribosomal 16S Environmental Microbiology Cluster Analysis Rhodococcus Rhodococcus sp Monooxygenase Biotransformation Phylogeny chemistry.chemical_classification 0303 health sciences BACTERIAL-DEGRADATION Strain (chemistry) GENUS RHODOCOCCUS General Medicine CHLOROPHENOL 4-MONOOXYGENASE TFTD Biochemistry Biodegradation Environmental Pollutants Energy source Metabolic Networks and Pathways Biotechnology DNA Bacterial Molecular Sequence Data OPACUS 1CP Biology DNA Ribosomal PSEUDOMONAS SP Microbiology 03 medical and health sciences Phenols 030304 developmental biology Portugal 030306 microbiology Substrate (chemistry) Metabolism Sequence Analysis DNA 16S ribosomal RNA 4-CHLOROPHENOL DEGRADATION FLUOROBENZOIC ACID Carbon 2-Fluorophenol (2-FP) Enzyme chemistry Luong model Energy Metabolism DEHALOGENATION |
| Zdroj: | Applied Microbiology and Biotechnology, 95(2), 511-520. SPRINGER Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0175-7598 |
| Popis: | A pure bacterial culture able to utilize 2- fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2- FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3- fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported. |
| Databáze: | OpenAIRE |
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