Protective Effects of Dexmedetomidine-Ketamine Combination Against Ventilator-Induced Lung Injury in Endotoxemia Rats
| Autor: | Chih Lin Yang, Tao Yeuan Wang, Chun Jen Huang, Cay Huyen Chen, Pei Shan Tsai |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide medicine.medical_treatment Anti-Inflammatory Agents Nitric Oxide Synthase Type II Pharmacology Lung injury Nitric Oxide Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Leukocytes medicine Animals Hypnotics and Sedatives Lung Tidal volume Peroxidase Ventilators Mechanical medicine.diagnostic_test biology business.industry Lung Injury respiratory system Endotoxemia Rats respiratory tract diseases Nitric oxide synthase Disease Models Animal medicine.anatomical_structure Bronchoalveolar lavage chemistry Immunology biology.protein Drug Therapy Combination Ketamine lipids (amino acids peptides and proteins) Surgery business Dexmedetomidine Prostaglandin E |
| Zdroj: | Journal of Surgical Research. 167:e273-e281 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2010.02.020 |
| Popis: | Background Pulmonary inflammatory response is crucial in mediating the development of ventilator-induced lung injury (VILI) in animals experiencing endotoxemia. Dexmedetomidine and ketamine are two sedative agents with potent anti-inflammatory capacity. We sought to elucidate the anti-inflammatory effects of dexmedetomidine-ketamine combination against VILI in endotoxemia rats. Materials and Methods Eighty-four adult male rats were allocated to receive normal saline, VILI, VILI plus dexmedetomidine-ketamine combination (D+K), lipopolysaccharide (LPS), LPS plus D+K, LPS plus VILI, or LPS plus VILI plus D+K (designated as the NS, V, V-D+K, LPS, LPS-D+K, LPS/V, and LPS/V-D+K group, respectively; n = 12 in each group). VILI was induced by high-tidal volume ventilation (tidal volume 20 mL/kg; respiratory rate 50 breath/min; FiO 2 21%). After being mechanically ventilated for 4 h, rats were sacrificed and the levels of pulmonary inflammatory response were evaluated. Results Histologic findings revealed severe, moderate, and mild inflammation in lung tissues of the LPS/V, LPS, and V groups, respectively, whereas those of the LPS/V-D+K, LPS-D+K, and V-D+K groups revealed moderate, mild, and normal to minimal inflammation, respectively. Moreover, the total cell number and the concentrations of macrophage inflammatory protein-2 and interleukin-1β in bronchoalveolar lavage fluid as well as the lung water content, leukocyte infiltration, myeloperoxidase activity, and the concentrations of inducible nitric oxide synthase/nitric oxide, and cyclooxygenase 2/prostaglandin E 2 in lung tissues of the LPS/V, LPS, and V groups were significantly higher than those of the LPS/V-D+K, LPS-D+K, and V-D+K groups, respectively. Conclusions Dexmedetomidine-ketamine combination could mitigate pulmonary inflammatory response induced by VILI in endotoxemia rats. |
| Databáze: | OpenAIRE |
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