Identification of relevant single-nucleotide polymorphisms in Pneumocystis jirovecii: relationship with clinical data
| Autor: | Kamal Mansinho, Francisco Antunes, T. Marques, Romulo Leite, Olga Matos, Francisco de Assis Esteves, Jorge F. Gaspar |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Microbiology (medical) RNA Mitochondrial DHPS Pneumocystis carinii Pneumocystis pneumonia Polymerase Chain Reaction Polymorphism Single Nucleotide Young Adult parasitic diseases Genotype medicine Humans pneumonia Pneumocystis jirovecii DNA Fungal Mycological Typing Techniques Aged Dihydropteroate Synthase AIDS-Related Opportunistic Infections Base Sequence Portugal biology Superoxide Dismutase Pneumonia Pneumocystis Haplotype Gene Amplification Fungal genetics Genetic Variation Sequence Analysis DNA General Medicine Cytochromes b Middle Aged medicine.disease biology.organism_classification Virology AIDS Tetrahydrofolate Dehydrogenase Infectious Diseases RNA Female clinical data Restriction fragment length polymorphism Dihydropteroate synthase SNPs |
| Zdroj: | Universidade Nova de Lisboa |
| ISSN: | 1198-743X |
| DOI: | 10.1111/j.1469-0691.2009.03030.x |
| Popis: | Pneumocystis jirovecii is a poorly understood pathogen that causes opportunistic pneumonia (Pneumocystis pneumonia (PcP)) in patients with AIDS. The present study was aimed at correlating genetic differences in P. jirovecii isolates and clinical patient data. A description of genetic diversity in P. jirovecii isolates from human immunodeficiency virus-positive patients, based on the identification of multiple single-nucleotide polymorphisms (SNPs) at five distinct loci encoding mitochondrial large-subunit rRNA (mtLSU rRNA), cytochrome b (CYB), superoxide dismutase (SOD), dihydrofolate reductase (DHFR), and dihydropteroate synthase (DHPS), was achieved using PCR with DNA sequencing and restriction fragment length polymorphism analysis. The statistical analysis revealed several interesting correlations among the four most relevant SNPs (mt85, SOD110, SOD215, and DHFR312) and specific clinical parameters: mt85C was associated with undiagnosed or atypical PcP episodes and favourable follow-up; SOD215C was associated with favourable follow-up; and DHFR312T was associated with PcP cases presenting moderate to high parasite burdens. The Geno-Types mt85C/SOD215C and SOD110T/SOD215C were found to be associated with less virulent P. jirovecii infections, whereas the Geno-Type SOD110T/SOD215T was found to be related to more virulent PcP episodes. The present work demonstrated that potential P. jirovecii haplotypes may be related to the clinical data and outcome of PcP. |
| Databáze: | OpenAIRE |
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