A transgenic mouse model for the detection of cellular stress induced by toxic inorganic compounds
| Autor: | Cinzia Parolini, Libero Clerici, Romeo Roncucci, Luigi Zecca, Luca Bagnasco, Enrica Mira Catò, Giulia Chiesa, Peter Bromley, Paolo Vezzoni, Maria Grazia Sacco |
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| Rok vydání: | 1997 |
| Předmět: |
Genetically modified mouse
Copper Sulfate Sodium arsenite Arsenites Transgene Biomedical Engineering Mice Transgenic Bioengineering Biology Cadmium chloride Polymerase Chain Reaction Applied Microbiology and Biotechnology Xenobiotics Mice chemistry.chemical_compound Cadmium Chloride Heat shock protein Animals Humans HSP70 Heat-Shock Proteins Transgenes Promoter Regions Genetic Cells Cultured Genetics Models Genetic Human Growth Hormone Mutagenicity Tests Methylmercury Compounds Sodium Compounds Molecular biology Hsp70 Gene Expression Regulation Liver chemistry Cell culture Toxicity Molecular Medicine Injections Intraperitoneal hormones hormone substitutes and hormone antagonists Biotechnology |
| Zdroj: | Nature Biotechnology. 15:1392-1397 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt1297-1392 |
| Popis: | Transgenic mice for genotoxicity testing have been developed, although no such models have been produced for the evaluation of toxic, nongenotoxic chemical compounds. We have developed a transgenic mouse model for the analysis of toxic inorganic compounds. We engineered a mouse lineage with the human growth hormone (hGH) gene under the control of the human hsp70 promoter, in which a plasma-detectable hGH response can be elicited by exposure to heat shock. In primary cell cultures from these mice, hGH release was observed following treatment with several toxic inorganics. Transgenic mice injected intraperitoneally with sodium arsenite, cadmium chloride, copper sulphate, or methylmercurium chloride showed significant hGH levels in plasma. |
| Databáze: | OpenAIRE |
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