Identification, characterization and microRNA expression profiling of side population cells in human oral squamous cell carcinoma Tca8113 cell lines
| Autor: | Xiang‑Pan Kong, Rong‑Sen Liu, Hong‑Chen Liu, Yang Bai, Wei Luo, Ling‑Ling E, Hao Wu, Hua‑Wei Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
cancer stem cells
0301 basic medicine Homeobox protein NANOG Cancer Research microRNA network Cell Biology Stem cell marker side population cells Biochemistry 03 medical and health sciences 0302 clinical medicine Side population Cancer stem cell Cell Line Tumor Genetics medicine Humans Tca8113 Side-Population Cells Molecular Biology Squamous Cell Carcinoma of Head and Neck Articles Cell cycle Molecular biology Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Oncology Head and Neck Neoplasms Cell culture 030220 oncology & carcinogenesis Molecular Medicine Stem cell Transcriptome |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | The present study aimed to evaluate the stem cell markers, characteristics and biological functions of cancer stem-like side population (SP) cells in human oral cancer. SP cells were isolated from the human oral squamous cell carcinoma Tca8113 cell line by Hoechst 33342 fluorescence dye and flow cytometry. The colony forming and proliferative capability of SP and non-SP cells were detected using a live-cell analysis system in vitro. The number of cells expressing stem cell markers was compared between SP cells and non-SP cells by flow cytometry. Reverse transcription-quantitative polymerase chain reaction and western blotting were used to detect the mRNA and protein expression levels of stem cell genes, respectively. Differential expression of microRNAs (miRNAs) in SP and non-SP cells was determined by microarray hybridization and an miRNA regulation network was produced. With regard to the proliferation capability, SP cells reached 60.0% confluence after 40 h of growth compared with 35.1% confluence for non-SP cells (P |
| Databáze: | OpenAIRE |
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