Synaptonemal Complex Components Are Required for Meiotic Checkpoint Function in Caenorhabditis elegans
| Autor: | Tisha Bohr, Kyra Firestone, Evan Eggleston, Guinevere Ashley, Needhi Bhalla |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell cycle checkpoint Chromosomal Proteins Non-Histone 1.1 Normal biological development and functioning Mutant Cell Cycle Proteins Investigations Biology 03 medical and health sciences checkpoint Meiosis Underpinning research Homologous chromosome Genetics Animals chromosome Caenorhabditis elegans Caenorhabditis elegans Proteins Synaptonemal Complex synapsis Synapsis Meiotic chromosome segregation Non-Histone biology.organism_classification Chromosomal Proteins Synaptonemal complex Chromosome Pairing 030104 developmental biology Mutation biological phenomena cell phenomena and immunity Developmental Biology |
| Zdroj: | Genetics, vol 204, iss 3 |
| Popis: | Synapsis involves the assembly of a proteinaceous structure, the synaptonemal complex (SC), between paired homologous chromosomes, and is essential for proper meiotic chromosome segregation. In Caenorhabditis elegans, the synapsis checkpoint selectively removes nuclei with unsynapsed chromosomes by inducing apoptosis. This checkpoint depends on pairing centers (PCs), cis-acting sites that promote pairing and synapsis. We have hypothesized that the stability of homolog pairing at PCs is monitored by this checkpoint. Here, we report that SC components SYP-3, HTP-3, HIM-3, and HTP-1 are required for a functional synapsis checkpoint. Mutation of these components does not abolish PC function, demonstrating they are bona fide checkpoint components. Further, we identify mutant backgrounds in which the instability of homolog pairing at PCs does not correlate with the synapsis checkpoint response. Altogether, these data suggest that, in addition to homolog pairing, SC assembly may be monitored by the synapsis checkpoint. |
| Databáze: | OpenAIRE |
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