Adenovirus polypeptide synthesis in the presence of non-replicating poliovirus
| Autor: | W. C. Russell, R. Bablanian |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Peptide Biosynthesis
Time Factors viruses Biology medicine.disease_cause Sulfur Radioisotopes Virus Replication Guanidines Virus Adenoviridae Cell Line chemistry.chemical_compound Methionine Transcription (biology) Virology medicine Guanidine Polyacrylamide gel electrophoresis DNA synthesis Cordycepin Poliovirus Carcinoma Cytarabine Sodium Dodecyl Sulfate Nucleosides Molecular biology Anti-Bacterial Agents chemistry Biochemistry Superinfection Autoradiography Electrophoresis Polyacrylamide Gel Mouth Neoplasms HeLa Cells |
| Zdroj: | The Journal of general virology. 24(2) |
| ISSN: | 0022-1317 |
| Popis: | Summary The effect of superinfection of adenovirus-infected cells with poliovirus in the presence of guanidine (P+G) has been analysed by pulse-labelling of cells with [35S]-methionine followed by polyacrylamide gel electrophoresis and autoradiography. The addition of P+G while reducing total polypeptide synthesis selectively inhibits cellular polypeptide synthesis and allows the visualization, particularly early in infection, of other labelled polypeptides previously obscured. Thus, at least three polypeptides i.e. ICSP-3, core-1 and ‘p-core 2’ can be detected in the absence of virus DNA synthesis. The addition of P+G also causes a retardation of the synthesis of the virus-induced polypeptides giving a clear sequence of translation viz ICSP-3: core-1: p-core 2 → ICSP-1 → hexon → penton base → fibre. Since a similar sequence can be demonstrated by the use of the inhibitor cordycepin, it seems possible that this reflects a sequence of transcription. P+G addition also effectively inhibits the formation of virus particles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|