Bilateral whisker trimming during early postnatal life impairs dendritic spine development in the mouse somatosensory barrel cortex

Autor: Dominique Muller, Mathias De Roo, Alexandre Dayer, Laszlo Vutskits, Adrian Briner, Jozsef Zoltan Kiss
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Yellow fluorescent protein
Genetically modified mouse
Dendritic spine
animal structures
Pyramidal Cells/metabolism/ultrastructure
Period (gene)
Dendritic Spines
Sensory system
Mice
Transgenic
Biology
Somatosensory system
Somatosensory Cortex/cytology/growth & development
03 medical and health sciences
Mice
Sensory Deprivation/ physiology
0302 clinical medicine
Animals
Dendritic spine head
Behavior
Animal/physiology
030304 developmental biology
0303 health sciences
Behavior
Animal
General Neuroscience
Pyramidal Cells
Age Factors
Somatosensory Cortex
Barrel cortex
Nerve Net/anatomy & histology/physiology
ddc:616.8
Luminescent Proteins/metabolism
Luminescent Proteins
Vibrissae/growth & development/ pathology
Vibrissae
biology.protein
Nerve Net
Sensory Deprivation
Dendritic Spines/physiology/ultrastructure
Neuroscience
030217 neurology & neurosurgery
Zdroj: Journal of Comparative Neurology, Vol. 518, No 10 (2010) pp. 1711-1723
The Journal of comparative neurology
ISSN: 0021-9967
Popis: The rodent somatosensory barrel cortex is an ideal model for studying the impact of sensory experience on developing brain circuitry. To examine whether and how interference with sensory perception in the early postnatal period can affect the development of synaptic networks in this system, we took advantage of a transgenic mouse strain expressing the yellow fluorescent protein in layer 5B pyramidal neurons of the somatosensory cortex. By using ex vivo confocal imaging, we first demonstrate a cortical-layer-specific increase in the number of dendritic spines during postnatal development on apical dendritic shafts of these cells extending up to cortical layer 1. Next, by performing bilateral whisker trimming at distinct developmental stages, we show that disruption of sensory perception before postnatal day 20 impairs dendritic spine development in apical dendritic segments within layers 1 and 2/3 but not in layer 4. The whisker trimming-induced decrease in dendritic spine density during this period is accompanied by a highly significant decrease in dendritic spine head diameter. Finally, we also show that these whisker trimming-induced morphological alterations of dendritic spines during the early postnatal period are no longer detectable in adult animals. Altogether, these findings further emphasize the important role of sensory activity in synaptic network assembly in the developing barrel cortex. They also support an as yet unidentified structural mechanism that might contribute to the layer- and cell-type-specific physiological effects of whisker trimming during the early postnatal period.
Databáze: OpenAIRE
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