A new pyruvate oxidase biosensor based on 3-mercaptopropionic acid/6-aminocaproic acid modified gold electrode
| Autor: | Ezgi Bayram, Erol Akyilmaz |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
3-Mercaptopropionic Acid
Pyruvate Oxidase Coefficient of variation Inorganic chemistry Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Biosensing Techniques chemistry.chemical_compound Monolayer Pyruvate oxidase Humans Electrodes Detection limit Electrochemical Techniques General Medicine Enzymes Immobilized chemistry Aminocaproic Acid Electrode Gold Glutaraldehyde Biosensor Biotechnology Nuclear chemistry |
| Zdroj: | Artificial Cells, Nanomedicine, and Biotechnology. 42:418-422 |
| ISSN: | 2169-141X 2169-1401 |
| DOI: | 10.3109/21691401.2013.815626 |
| Popis: | In the biosensor construction, 3-mercaptopropionic acid (3-MPA) and 6-aminocaproic acid (6-ACA) were used for forming self-assembled monolayer (SAM) on a gold disc electrode and pyruvate oxidase was immobilized on the modified electrode surface by using glutaraldehyde. Biosensor response is linearly related to pyruvate concentration at 2.5-50 μM, detection limit is 1.87 μM and response time of the biosensor is 6 s for differential pulse voltammograms. From the repeatability studies (n = 6) for 30.0 μM pyruvate revealed that the average value ([Formula: see text]), standard deviation (S.D) and coefficient of variation (CV %) were calculated to be 31.02 μM, ± 0.1914 μM and 0.62%, respectively. |
| Databáze: | OpenAIRE |
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