Downregulation of plasma microRNA-29c-3p expression may be a new risk factor for diabetic retinopathy
| Autor: | Mehmet Argun, Levent Tok, Hakan Korkmaz, Sonmez Şevi K, Bora Torus, Fevziye Burcu Sirin, Kuyaş Hekimler Öztürk |
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| Rok vydání: | 2023 |
| Předmět: |
medicine.medical_specialty
business.industry Endocrinology Diabetes and Metabolism Diabetic retinopathy Fundus (eye) medicine.disease Gastroenterology Vascular endothelial growth factor chemistry.chemical_compound Real-time polymerase chain reaction Endocrinology chemistry Downregulation and upregulation Internal medicine Diabetes mellitus medicine Internal Medicine Risk factor business Retinopathy |
| Zdroj: | Minerva Endocrinology. 48 |
| ISSN: | 2724-6116 2724-6507 |
| Popis: | Background Circulation miRNAs have emerged as new biomarkers for identifying and monitoring the microvascular complications of diabetes. The aim of this study is to evaluate the levels of five candidate miRNAs (miR-29c-3p, miR-18a, miR-31, miR-181 and miR-20a) in patients with diabetic retinopathy (DR) and their relationship with disease severity. Methods The study included 31 diabetes patients without DR (NDR group), 68 patients with DR (DR group) and 30 healthy controls (HC group). Twenty-five of patients with DR were proliferative DR (PDR group) and 43 were non-proliferative DR (NPDR group) patients. Metabolic parameters and serum vascular endothelial growth factor (VEGF) levels of all participants were measured. Circulating miRNAs levels were determined by quantitative realtime PCR. Fundus examinations of all patients were performed by a single ophthalmologist. Results VEGF levels were significantly higher in the NDR and DR groups compared to HC group (p=0.011 and p=0.014, respectively). Plasma miR-29c-3p was downregulated in diabetic patients with retinopathy and without retinopathy. This downregulation was more prominent in diabetic patients without retinopathy compared to those with retinopathy (p=0.016). There was no significant difference in plasma levels of miR-18a, miR-20a, miR-18a and miR-31 between diabetic subjects with and without retinopathy (p>0.05). There was no correlation between DR severity and the levels of miRNAs (p>0.05). In multivariate logistic regression analysis, it was found that changes in plasma miR-29c-3p expression of diabetic patients increased DR risk independent of other risk factors. Conclusions Plasma miR-29c-3p expression is downregulated in diabetic patients with and without retinopathy, and changes in this miRNA are an independent risk factor for the development of DR. |
| Databáze: | OpenAIRE |
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