Pro-Apoptotic and Anti-Invasive Properties Underscore the Tumor-Suppressing Impact of Myoglobin on a Subset of Human Breast Cancer Cells
| Autor: | Aboouf, Mostafa A, Armbruster, Julia, Thiersch, Markus, Guscetti, Franco, Kristiansen, Glen, Schraml, Peter, Bicker, Anne, Petry, Ruben, Hankeln, Thomas, Gassmann, Max, Gorr, Thomas A |
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| Přispěvatelé: | University of Zurich, Gorr, Thomas A |
| Rok vydání: | 2022 |
| Předmět: |
Epithelial-Mesenchymal Transition
1503 Catalysis 10184 Institute of Veterinary Pathology 1607 Spectroscopy Breast Neoplasms Catalysis Inorganic Chemistry Cell Line Tumor Cyclins 10049 Institute of Pathology and Molecular Pathology 1312 Molecular Biology 1706 Computer Science Applications Humans 11434 Center for Clinical Studies Physical and Theoretical Chemistry Molecular Biology Spectroscopy Myoglobin 1604 Inorganic Chemistry Organic Chemistry General Medicine 10081 Institute of Veterinary Physiology tumorigenesis migration apoptosis ROS estrogen receptor hypoxia chemotherapy radiation p53 Computer Science Applications Oxygen Doxorubicin 10076 Center for Integrative Human Physiology 570 Life sciences biology Female Tumor Suppressor Protein p53 1606 Physical and Theoretical Chemistry 1605 Organic Chemistry |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11483 |
| DOI: | 10.5167/uzh-222284 |
| Popis: | The expression of myoglobin (MB), well known as the oxygen storage and transport protein of myocytes, is a novel hallmark of the luminal subtype in breast cancer patients and correlates with better prognosis. The mechanisms by which MB impacts mammary tumorigenesis are hitherto unclear. We aimed to unravel this role by using CRISPR/Cas9 technology to generate MB-deficient clones of MCF7 and SKBR3 breast cancer cell lines and subsequently characterize them by transcriptomics plus molecular and functional analyses. As main findings, loss of MB at normoxia upregulated the expression of cell cyclins and increased cell survival, while it prevented apoptosis in MCF7 cells. Additionally, MB-deficient cells were less sensitive to doxorubicin but not ionizing radiation. Under hypoxia, the loss of MB enhanced the partial epithelial to mesenchymal transition, thus, augmenting the migratory and invasive behavior of cells. Notably, in human invasive mammary ductal carcinoma tissues, MB and apoptotic marker levels were positively correlated. In addition, MB protein expression in invasive ductal carcinomas was associated with a positive prognostic value, independent of the known tumor suppressor p53. In conclusion, we provide multiple lines of evidence that endogenous MB in cancer cells by itself exerts novel tumor-suppressive roles through which it can reduce cancer malignancy. |
| Databáze: | OpenAIRE |
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