Ibuprofen blocks changes in Na v 1.7 and 1.8 sodium channels associated with complete Freund's adjuvant-induced inflammation in rat
Autor: | R. Denis Soignier, Dennis Paul, Z.P Liu, S. Rock Levinson, Harry J. Gould, Lerna D Minor, Porter Nolan, John D. England |
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Rok vydání: | 2004 |
Předmět: |
Freund's Adjuvant
Stimulation Ibuprofen Nerve Tissue Proteins Pharmacology Sodium Channels Cyclooxygenase pathway NAV1.8 Voltage-Gated Sodium Channel Rats Sprague-Dawley Subcutaneous injection Dorsal root ganglion medicine Animals Cyclooxygenase Inhibitors Pain Measurement Inflammation biology business.industry Sodium channel NAV1.7 Voltage-Gated Sodium Channel Rats Up-Regulation Anesthesiology and Pain Medicine medicine.anatomical_structure Nerve growth factor Neurology Anesthesia Hyperalgesia biology.protein Neurology (clinical) Cyclooxygenase medicine.symptom business Sodium Channel Blockers |
Zdroj: | The journal of pain. 5(5) |
ISSN: | 1526-5900 |
Popis: | Although nerve growth factor plays a role in augmenting sodium channel expression in small dorsal root ganglion (DRG) cells, the cytochemical mediators responsible for enhanced expression in large DRG neurons are unknown. To narrow the search for mediators involved in the increased production of sodium channels in large DRG neurons, we examined the effect of cyclooxygenase inhibition on sodium channel production during inflammation. Thirty minutes before the subcutaneous injection of complete Freund's adjuvant (CFA), rats received ibuprofen (nonselective, cyclooxygenase inhibitor), NS-398 (selective, cyclooxygenase inhibitor), or vehicle. Withdrawal thresholds from thermal and mechanical stimulation were measured before and immediately after CFA injection and at selected hourly intervals after injection for the next 24 hours. Sodium channel up-regulation was then examined in DRG by using site-specific, anti–sodium channel antibodies, Na v 1.7 and 1.8. Both ibuprofen and NS-398 provided analgesia during the second phase of inflammatory hyperalgesia that begins 3 hours after CFA injection. The up-regulation, predominantly of Na v 1.7 and minimally of Na v 1.8 channels, seen in vehicle-treated rats was suppressed by both drugs at 24 hours after injection. By 72 hours after injection, no difference in labeling between the drug- and vehicle-treated animals was observed. Sodium channel labeling in large DRG neurons returned to baseline between 1 and 2 weeks after CFA injection, whereas small cell labeling persisted. The cytochemical signal for sodium channel up-regulation in the large DRG cells that most closely correlates with inflammatory hyperalgesia is mediated at least in part through products of the cyclooxygenase pathway. Perspective Expression of sodium channels in dorsal root ganglia increases dramatically during inflammation. The increase in sodium channels is thought to enhance neuronal excitability and to play a role in hyperalgesia and wound vigilance during healing. We provide evidence that prostaglandins play a role in signaling channel augmentation. |
Databáze: | OpenAIRE |
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