Gut microbiome composition and risk factors in a large cross-sectional IBS cohort

Autor: Lauren N Carroll, Ignacio Varas, Nabeel Imam, Claire Greene, Rodolfo Pino, Daniel Almonacid, Christina Palmer, Sarah Gupta, Melissa Agnello, Maureen Hitschfeld, Michael C. Hoaglin
Rok vydání: 2020
Předmět:
Adult
Male
0301 basic medicine
medicine.medical_specialty
intestinal microbiology
Comorbidity
genetic testing
Cohort Studies
Irritable Bowel Syndrome
Feces
03 medical and health sciences
0302 clinical medicine
Risk Factors
RNA
Ribosomal
16S
Surveys and Questionnaires
Internal medicine
medicine
Humans
molecular biology
Clinical significance
Microbiome
lcsh:RC799-869
Family history
stool markers
Irritable bowel syndrome
Genetic testing
medicine.diagnostic_test
business.industry
Microbiota
Confounding
Gastroenterology
Middle Aged
Classification
medicine.disease
Anti-Bacterial Agents
Gastrointestinal Microbiome
Cross-Sectional Studies
Logistic Models
030104 developmental biology
Gastrointestinal disorder
Case-Control Studies
Cohort
Dysbiosis
lcsh:Diseases of the digestive system. Gastroenterology
Female
030211 gastroenterology & hepatology
Self Report
business
Zdroj: BMJ Open Gastroenterology
BMJ Open Gastroenterology, Vol 7, Iss 1 (2020)
ISSN: 2054-4774
Popis: ObjectiveIrritable bowel syndrome (IBS) is a common gastrointestinal disorder that is difficult to diagnose and treat due to its inherent heterogeneity and unclear aetiology. Although there is evidence suggesting the importance of the microbiome in IBS, this association remains poorly defined. In the current study, we aimed to characterise a large cross-sectional cohort of patients with self-reported IBS in terms of microbiome composition, demographics, and risk factors.DesignIndividuals who had previously submitted a stool sample for 16S microbiome sequencing were sent a comprehensive survey regarding IBS diagnosis, demographics, health history, comorbidities, family history, and symptoms. Log ratio-transformed abundances of microbial taxa were compared between individuals reporting a diagnosis of IBS without any comorbidities and individuals reporting no health conditions. Univariable testing was followed by a multivariable logistic regression model controlling for relevant confounders.ResultsOut of 6386 respondents, 1692 reported a diagnosis of IBS without comorbidities and 1124 reported no health conditions. We identified 3 phyla, 15 genera, and 19 species as significantly associated with IBS after adjustment for confounding factors. Demographic risk factors include a family history of gut disorders and reported use of antibiotics in the last year.ConclusionThe results of this study confirm important IBS risk factors in a large cohort and support a connection for microbiome compositional changes in IBS pathogenesis. The results also suggest clinical relevance in monitoring and investigating the microbiome in patients with IBS. Further, the exploratory models described here provide a foundation for future studies.
Databáze: OpenAIRE
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