Germ plasm provides clues on meiosis: the concerted action of germ plasm granules and mitochondria in gametogenesis of the clam Ruditapes philippinarum
| Autor: | Arkadiy Reunov, Yulia Reunova, Liliana Milani, Yana N. Alexandrova, Alina Komkova |
|---|---|
| Přispěvatelé: | Reunov, Arkadiy, Alexandrova, Yana, Reunova, Yulia, Komkova, Alina, Milani, Liliana |
| Rok vydání: | 2018 |
| Předmět: |
Male
Manila clam Reproductive technology Biology Germ plasm-related structures (GPRS) Oogenesis Balbiani Body Germline 03 medical and health sciences 0302 clinical medicine Meiosis Meiotic differentiation Ruditapes philippinarum Testis Animals CYTB Spermatogenesis Gametogenesis Germ plasm Organelles 030219 obstetrics & reproductive medicine urogenital system Ovary 0402 animal and dairy science Gametogenesi 04 agricultural and veterinary sciences Cell Biology 040201 dairy & animal science Spermatozoa Cell biology Chromatin Bivalvia Mitochondria Oocytes VASA Female Developmental Biology |
| Zdroj: | Zygote (Cambridge, England). 27(1) |
| ISSN: | 1469-8730 |
| Popis: | SummaryGerm plasm-related structures (GPRS) are known to accompany meiotic cell differentiation but their dynamics are still poorly understood. In this study, we analyzed the ultrastructural mechanisms of GPRS transformation during oogenesis and spermatogenesis of the bivalve molluscRuditapes philippinarum(Manila clam), exploring patterns of GPRS activity occurring at meiosis onset, sex-specific difference/similarity of such patterns, and the involvement of mitochondria during GPRS-assigned events. In the two sexes, the zygotene–pachytene stage of meiosis is anticipated by three shared steps. First, the dispersion of germ plasm granules containing the germ line determinant VASA occurs. Second, the VASA protein deriving from germ plasm granules enters neighbouring mitochondria and appears to induce mitochondrial matter release, as supported by cytochrome B localization outside the mitochondria. Third, intranuclear VASA entrance occurs and the protein appears involved in chromatin reorganization, as supported by VASA localization in synaptonemal complexes. In spermatogenesis, these three steps are sufficient for the normal course of meiosis. In oogenesis, these are followed by the action of ‘germ plasm granule formation complex’, a novel type of structure that appears alternative to the Balbiani body. The possibility of germ plasm involvement in reproductive technologies is also suggested. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|