Regulation of the Xenopus Xsox17α1 promoter by co-operating VegT and Sox17 sites
| Autor: | Maria Rex, Hugh R. Woodland, Debbie Clements, Laura Howard |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
TGF-β
SOXF Transcription Factors animal structures Xenopus Transgenic embryo Xenopus Proteins Biology Sox17 Xenopus laevis 03 medical and health sciences Transcription (biology) medicine Animals Promoter Regions Genetic Gene Transcription factor Molecular Biology 030304 developmental biology VegT 0303 health sciences urogenital system 030302 biochemistry & molecular biology Endoderm High Mobility Group Proteins Gene Expression Regulation Developmental Promoter Cell Biology Blastula biology.organism_classification Molecular biology DNA-Binding Proteins Gastrulation medicine.anatomical_structure Genomes & Developmental Control Mutation embryonic structures T-Box Domain Proteins Transcription Factors Developmental Biology |
| Zdroj: | Developmental Biology |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2007.07.028 |
| Popis: | The gene encoding the Sox F-group transcription factor Xsox17alpha(1) is specifically expressed throughout the entire region of the Xenopus blastula fated to become endoderm, and is important in controlling endodermal development. Xsox17alpha(1) is a direct target of the maternal endodermal determinant VegT and of Sox17 itself. We have analysed the promoter of the Xenopus laevis Xsox17alpha(1) gene by transgenesis, and have identified two important control elements which reside about 9 kb upstream at the start of transcription. These elements individually drive transgenic endodermal expression in the blastula and gastrula. One contains functional, cooperating VegT and Sox-binding consensus sites. The Sox sites in this region are occupied in vivo. The other responds to TGF-beta signals like Activin or Nodals that act through Smad2/3. We propose that these two regions co-operate in regulating the early endodermal expression of the Xsox17alpha(1) gene. |
| Databáze: | OpenAIRE |
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