Salispheres from Different Major Salivary Glands for Glandular Regeneration
| Autor: | Tsung-Lin Yang, Hsiao-Fen Lee, Tai-Horng Young, Yee-Chun Chen, Y C Hsiao |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Saliva Submandibular Gland Saliva secretion Biology Salivary Glands Cell therapy Sublingual Gland 03 medical and health sciences 0302 clinical medicine stomatognathic system In vivo Major Salivary Gland Animals Parotid Gland Regeneration Progenitor cell General Dentistry Cells Cultured Stem Cells Regeneration (biology) Cell biology Mice Inbred C57BL 030104 developmental biology 030220 oncology & carcinogenesis Stem cell |
| Zdroj: | Journal of Dental Research. 98:786-794 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/0022034519847122 |
| Popis: | Dysfunctional salivary glands (SGs) are a clinical challenge due to the lack of effective treatments. Cell therapy with stem/progenitor cells may improve this situation by providing promising therapeutic solutions. Therefore, exploring abundant cellular sources is important. Three major pairs of SGs are located in different anatomic regions: the parotid glands, the submandibular glands, and the sublingual glands. Although SG stem/progenitor cells can be isolated and cultivated from all major SGs as salispheres, the differences among SG origins remain unclear. In this study, salispheres were successfully isolated from all major SGs. The salispheres demonstrated unique cellular features that originated from their native tissues. The characteristic expression profiles and cellular features of SG stem cells were demonstrated in all salispheres. When they were transplanted into irradiated animals, the salispheres were all capable of improving the saliva secretion that was disrupted by irradiation. Typical histologic structures could be observed in most parts of the treated glands, and the fibrotic environments of irradiated submandibular glands were remodeled by all salispheres regardless of origins. This study characterized the cellular features and in vivo effects of salispheres that were derived from different anatomic origins. The results suggest the possibility of functional redundancy among distinct pairs of major SGs, which is useful for the design of cell therapy to treat dysfunctional glandular organs. |
| Databáze: | OpenAIRE |
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