Visualizing the Neutrophil Response to Sterile Tissue Injury in Mouse Dermis Reveals a Three-Phase Cascade of Events
Autor: | Ben Roediger, Lai Guan Ng, Yilin Wang, Michael John de Veer, Michele A. Grimbaldeston, Jim S. Qin, Lois L. Cavanagh, Els N.T. Meeusen, Cheryl A Jones, Rohit Jain, Wolfgang Weninger, Adrian Smith |
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Přispěvatelé: | Ng, Lai Guan, Qin, Jim S, Roediger, Ben, Wang, Yilin, Jain, Rohit, Cavanagh, Lois L, Smith, Adrian L, Jones, Cheryl A, de Veer, Michael, Grimbaldeston, Michele A, Meeusen, Els N, Weninger, Wolfgang |
Rok vydání: | 2011 |
Předmět: |
Pathology
medicine.medical_specialty mice Neutrophils Green Fluorescent Proteins Inflammation immunosurveillance Dermatology Biology Pertussis toxin Biochemistry organ injury 03 medical and health sciences Mice 0302 clinical medicine Interstitial space Dermis neutrophil granulocytes Cell Movement medicine Animals tissue repair Cell damage Molecular Biology 030304 developmental biology Skin 0303 health sciences Cyclic ADP-Ribose Microscopy Wound Healing Sheep tissue damage Neutrophil extracellular traps Cell Biology medicine.disease Flow Cytometry dermis Immunosurveillance Mice Inbred C57BL medicine.anatomical_structure Pertussis Toxin medicine.symptom Wound healing pathogen clearance 030217 neurology & neurosurgery |
Zdroj: | Journal of Investigative Dermatology. 131(10):2058-2068 |
ISSN: | 0022-202X |
DOI: | 10.1038/jid.2011.179 |
Popis: | Neutrophil granulocytes traffic into sites of organ injury in which they may not only participate in tissue repair and pathogen clearance but may also contribute to collateral cell damage through the release of noxious mediators. The dynamics and mechanisms of neutrophil migration in the extravascular space toward loci of tissue damage are not well understood. Here, we have used intravital multi-photon microscopy to dissect the behavior of neutrophils in response to tissue injury in the dermis of mice. We found that, following confined physical injury, initially rare scouting neutrophils migrated in a directional manner toward the damage focus. This was followed by the attraction of waves of additional neutrophils, and finally stabilization of the neutrophil cluster around the injury. Although neutrophil migration in the steady state and during the scouting phase depended on pertussis toxin-sensitive signals, the amplification phase was sensitive to interference with the cyclic adenosine diphosphate ribose pathway. We finally demonstrated that neutrophil scouts also transit through the non-inflamed dermis, suggesting immunosurveillance function by these cells. Together, our data unravel a three-step cascade of events that mediates the specific accumulation of neutrophils at sites of sterile tissue injury in the interstitial space. Refereed/Peer-reviewed |
Databáze: | OpenAIRE |
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