Photodynamic inactivation of methicillin-resistant Staphylococcus aureus and Escherichia coli: A metalloporphyrin comparison
| Autor: | Matthew A. Bork, Troy Skwor, Stephanie A. Blaszczyk, Brianna Schardt, Stephanie Klemm, Hanyu Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine 030103 biophysics Metalloporphyrins medicine.drug_class Antibiotics Biophysics PDI MRSA medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Escherichia coli medicine Radiology Nuclear Medicine and imaging Photosensitizer chemistry.chemical_classification Reactive oxygen species Photosensitizing Agents Radiation Radiological and Ultrasound Technology Singlet oxygen E. coli Ligand (biochemistry) Porphyrin Anti-Bacterial Agents Metalloporphyrin Photochemotherapy chemistry Staphylococcus aureus Radiology Nuclear Medicine and imaging TMPyP |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 165:51-57 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/j.jphotobiol.2016.10.016 |
| Popis: | Increasing rates of antibiotic resistance coupled with the lack of novel antibiotics threatens proper clinical treatment and jeopardizes their use in prevention. A photodynamic approach appears to be an innovative treatment option, even for multi-drug resistant strains of bacteria. Three components are utilized in photodynamic inactivation: a photosensitizer, light source, and oxygen. Variations in photosensitizers strongly influence microbial binding and bactericidal activity. In this study, four different cationic metalloporphyrins (Cu2 +, Fe2 +, Pd2 +, Zn2 +) were compared to the free-base ligand 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin regarding their electronic properties and generation of reactive oxygen species upon subsequent 405 nm violet-blue irradiation. Staphylococcus aureus and Escherichia coli were used as representatives of Gram-positive and -negative, respectively, to assess bactericidal effects by the photodynamic process. Bacterial cultures were pre-incubated with porphyrins and exposed to varying doses of 405 nm irradiation (0–30 J/cm2). Metalloporphyrins containing Cu2 + and Fe2 + demonstrated minimal effects on viability. Pronounced bactericidal activity was evident with free-base ligand, Zn2 +, and Pd2 +; though significantly stronger effects were apparent with Pd2 +. Photodynamic killing was directly proportional to reactive oxygen species production post-illumination. These data provide new insight into the influence of metal chelation on photosensitizer activity on bactericidal singlet oxygen production. The strong anti-microbial photodynamic action through the use of a portable light-emitting diode over short time intervals (seconds) provides support for its potential use in self-treatment. |
| Databáze: | OpenAIRE |
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