Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines
| Autor: | Ann M. Dvorak, Stephen J. Galli, H. W. Rogers, S D Wilson, C. A. Martin, Sundararajan Jayaraman, John R. Gordon, P R Burd, Martin E. Dorf |
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| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_treatment
Immunology Receptors Fc Immunoglobulin E Cell Line Biological Factors Mice Antigen medicine Animals Immunology and Allergy Mast Cells Interleukin 5 Cells Cultured Interleukin 4 Mice Inbred BALB C biology Receptors IgE Nucleic Acid Hybridization Articles Blotting Northern Mast cell Molecular biology Clone Cells Antigens Differentiation B-Lymphocyte Mice Inbred C57BL Interleukin 33 Cytokine medicine.anatomical_structure Gene Expression Regulation biology.protein Cytokines RNA Interleukin-3 Antibody DNA Probes |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | In response to IgE and specific multivalent antigen, mast cell lines (both growth factor-dependent and -independent) induce the transcription and/or secretion of a number of cytokines having a wide spectrum of activities. We have identified IL-1, IL-3, IL-5, IL-6, IFN-gamma, GM-CSF, JE, MIP1 alpha, MIP1 beta, and TCA3 RNA in at least two of four mast cell clones. The production of these products (except JE) is activation-associated and can be induced by IgE plus antigen. In selected instances cytokine expression can also be induced by activation with Con A or phorbol ester plus ionophore, albeit to levels less than those observed with IgE plus antigen. In addition, long-term mast cell clones and primary cultures of bone marrow-derived mast cells specifically release IL-1, IL-4, and/or IL-6 bioactivity after activation. These findings suggest that in addition to their inflammatory effector function mast cells may serve as a source of growth and regulatory factors. The relationship of mast cells to cells of the T lymphocyte lineage is discussed. |
| Databáze: | OpenAIRE |
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