Bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and vital for their genomic targeting in Arabidopsis
| Autor: | Tao Zhu, Xin Song, Jianqu Xu, Yuhui Liang, Zhenwei Liang, Yawen Lei, Chenlong Li, Wei Fu, Yaoguang Yu, Yuanhao Hao, Liangbing Yuan |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chromosomal Proteins Non-Histone cells Protein subunit genetic processes Arabidopsis macromolecular substances Plant Science Biology 01 natural sciences Chromatin remodeling 03 medical and health sciences Gene Expression Regulation Plant Molecular Biology Gene Adenosine Triphosphatases Arabidopsis Proteins Gene Expression Profiling Gene targeting biology.organism_classification Chromatin Assembly and Disassembly SWI/SNF Chromatin Cell biology Bromodomain enzymes and coenzymes (carbohydrates) 030104 developmental biology biological phenomena cell phenomena and immunity 010606 plant biology & botany Transcription Factors |
| Zdroj: | Molecular plant. 14(6) |
| ISSN: | 1752-9867 |
| Popis: | Switch defective/sucrose non-fermentable (SWI/SNF) chromatin remodeling complexes are multi-subunit machines that play vital roles in the regulation of chromatin structure and gene expression. However, the mechanisms by which SWI/SNF complexes recognize their target loci in plants are not fully understood. Here, we show that the Arabidopsis thaliana bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and critical for SWI/SNF genomic targeting. These three BRDs interact directly with multiple SWI/SNF subunits, including the BRAHMA (BRM) catalytic subunit. Phenotypic and transcriptomic analyses of the brd1 brd2 brd13 triple mutant revealed that these BRDs act largely redundantly to control gene expression and developmental processes that are also regulated by BRM. Genome-wide occupancy profiling demonstrated that these three BRDs extensively colocalize with BRM on chromatin. Simultaneous loss of function of three BRD genes results in reduced BRM protein levels and decreased occupancy of BRM on chromatin across the genome. Furthermore, we demonstrated that the bromodomains of BRDs are essential for genomic targeting of the BRD subunits of SWI/SNF complexes to their target sites. Collectively, these results demonstrate that BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and reveal their biological roles in facilitating genomic targeting of BRM-containing SWI/SNF complexes in plants. |
| Databáze: | OpenAIRE |
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