Gut ischemia-reperfusion affects gut mucosal immunity: A possible mechanism for infectious complications after severe surgical insults*
| Autor: | Chikara Ueno, Shuri Sakamoto, Kazuhiko Fukatsu, Hoshio Hiraide, Yoshinori Maeshima, Hidetaka Mochizuki, Isamu Matsumoto, Etsuko Hara |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Immunoglobulin A Resuscitation Lymphoid Tissue Gut-associated lymphoid tissue Ischemia Mice Inbred Strains Critical Care and Intensive Care Medicine Mice Peyer's Patches Random Allocation Postoperative Complications Reference Values Immunity Intensive care Intestine Small medicine Animals Lymphocyte Count Intestinal Mucosa Cells Cultured Probability Laparotomy biology business.industry Bacterial Infections Flow Cytometry medicine.disease Disease Models Animal medicine.anatomical_structure Lymphatic system Reperfusion Injury Immunology biology.protein Complication business |
| Zdroj: | Critical Care Medicine. 34:182-187 |
| ISSN: | 0090-3493 |
| DOI: | 10.1097/01.ccm.0000196207.86570.16 |
| Popis: | To examine influences of gut ischemia/reperfusion (I/R) on gut-associated lymphoid tissue (GALT) mass and function.Prospective, randomized controlled study.Research laboratory.Male Institute of Cancer Research mice.Ninety mice were randomized to three groups: I/R (60-min gut ischemia), sham (laparotomy only), and control (no operation). On days 1, 2, 4, 7, and 10, mice were killed to harvest lymphocytes from Peyer patches, the intraepithelial space, and the lamina propria (LP) of the small intestine. Respiratory tract and small intestinal washings were also obtained.Gut I/R significantly reduced lymphocyte numbers in Peyer patches, the intraepithelial space, and the LP. The reduction was prominent in GALT effector sites, that is, the intraepithelial space and LP, but numbers recovered quickly in LP. Changes in cell numbers in Peyer patches, GALT inductive sites, were subtle but persistent. Gut I/R reduced B cell numbers in Peyer patches; alphabeta T cell receptor (TCR)+, gammadeltaTCR+, CD8+, and B cell numbers in the intraepithelial space; and gammadeltaTCR+, CD8+, and B cell numbers in the LP, in comparison with the sham or control group. There were no significant differences in respiratory tract immunoglobulin A levels between the I/R and sham groups. Intestinal immunoglobulin A was elevated on day 1 in the I/R group, with no significant difference after day 2 in comparison with the sham group.Despite the maintained mucosal immunoglobulin A level, gut I/R markedly reduces GALT cell numbers, with changes in lymphocyte phenotypes. These alterations may be associated with increased morbidity due to infectious complications after severe surgical insults. |
| Databáze: | OpenAIRE |
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