Ovarian Stimulation Affects the Population of Mouse Uterine NK Cells at Early Pregnancy
| Autor: | Seyed Mohammad Moazzeni, Parvin Dorfeshan, Mojdeh Salehnia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Stromal cell Article Subject medicine.medical_treatment Population Uterus lcsh:Medicine Pregnant Mare Serum Gonadotropin Biology Endometrium Chorionic Gonadotropin General Biochemistry Genetics and Molecular Biology Human chorionic gonadotropin Mice Ovulation Induction Pregnancy Internal medicine medicine Animals Humans Embryo Implantation education Progesterone education.field_of_study General Immunology and Microbiology Estradiol lcsh:R Myometrium General Medicine Killer Cells Natural medicine.anatomical_structure Endocrinology Ovulation induction Female Stromal Cells Spleen Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2013 (2013) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | The aim of this study was to determine the influence of ovarian stimulation on endometrial mouse NK cell population. For superovulation, the female adult NMRI mice were injected i.p. with 10 IU of the pregnant mare serum gonadotropin followed 48 h later by an i.p. injection of 10 IU human chorionic gonadotropin hormone. Ovarian stimulated and nonstimulated mice were mated with fertile male. The presence of vaginal plug proved natural pregnancy, and this day was considered as day one of pregnancy. Tissue samples were prepared from the uterine horn and spleen of both groups of study on 7th day of pregnancy. Serum estradiol-17βand progesterone were measured at the same time. The tissue cryosections were prepared and double stained for CD 161 and CD3 markers, and NK cells population was analyzed. Relative frequency of NK cells was significantly lower in stroma and myometrium in hyperstimulated mice compared with the control group. However, no difference was seen in percentage of NK cells in spleen. The ovarian stimulation influences the proportion of uterine NK cells and may affect the embryo implantation. |
| Databáze: | OpenAIRE |
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