Genome-wide phenotypic RNAi screen in the Drosophila wing: phenotypic description of functional classes
| Autor: | Cristina M. Ostalé, Mercedes Martín, Cristina Molnar, Nuria Esteban, Covadonga F Hevia, María F. Organista, Ana López-Varea, Patricia Vega-Cuesta, Jose F. de Celis, Joaquín Culí, Ana Ruiz-Gómez, Jesús de Celis |
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| Přispěvatelé: | UAM. Departamento de Biología Molecular |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI01140
animal structures AcademicSubjects/SCI00010 screen Computational biology Biology QH426-470 AcademicSubjects/SCI01180 Genome RNA interference Genetics Animals Drosophila Proteins Wings Animal Drosophila (subgenus) wing Molecular Biology Gene Genetics (clinical) Gene knockdown Mutant Screen Report Biología y Biomedicina / Biología biology.organism_classification Phenotype Metabolism RNAi AcademicSubjects/SCI00960 Drosophila RNA Interference Signal transduction Drosophila Protein |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 11, Iss 12 (2021) G3: Genes|Genomes|Genetics Biblos-e Archivo. Repositorio Institucional de la UAM instname |
| ISSN: | 2160-1836 |
| Popis: | The Drosophila genome contains approximately 14,000 protein-coding genes encoding all the necessary information to sustain cellular physiology, tissue organization, organism development, and behavior. In this manuscript, we describe in some detail the phenotypes in the adult fly wing generated after knockdown of approximately 80% of Drosophila genes. We combined this phenotypic description with a comprehensive molecular classification of the Drosophila proteins into classes that summarize the main expected or known biochemical/functional aspect of each protein. This information, combined with mRNA expression levels and in situ expression patterns, provides a simplified atlas of the Drosophila genome, from housekeeping proteins to the components of the signaling pathways directing wing development, that might help to further understand the contribution of each gene group to wing formation. |
| Databáze: | OpenAIRE |
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