Characterization of a nanoflow sheath liquid interface and comparison to a sheath liquid and a sheathless porous-tip interface for CE-ESI-MS in positive and negative ionization
| Autor: | Cristina Montealegre, Oliver Höcker, Christian Neusüß |
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| Rok vydání: | 2018 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Analyte Materials science Capillary action Electrospray ionization 02 engineering and technology Mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry Capillary electrophoresis Ionization Common emitter Syringe driver business.industry 010401 analytical chemistry Antibodies Monoclonal Electrophoresis Capillary Equipment Design 021001 nanoscience & nanotechnology 0104 chemical sciences Optoelectronics Peptides 0210 nano-technology business Porosity |
| Zdroj: | Analytical and Bioanalytical Chemistry. 410:5265-5275 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-018-1179-3 |
| Popis: | Coupling of capillary electrophoresis to electrospray mass spectrometry still remains challenging and a topic of research to find the best interface regarding sensitivity, robustness, and ease of use. Here, a nanoflow sheath liquid interface for CE-ESI-MS is presented and compared to both a standard triple-tube sheath liquid and a porous-tip sheathless interface for three groups of analytes. The nanoflow sheath liquid interface with a separation capillary inserted into a glass emitter was initially characterized to facilitate optimization and method development. Implementation of a shut-off valve, syringe pump, and inline filter enabled easy handling and fast analyses, repeatable both in positive and negative modes (intra-day RSD of 6.6 to 12.0%). The same setup was used for sheathless interfacing by exchanging the emitter and using a porous etched tip separation capillary. Both nanoflow interfaces showed similar performance. Average peak areas using the nanoflow sheath liquid interface were a factor of 38 for 6 organic acids in negative mode, 114 and 36 for the light and heavy chain of a monoclonal antibody, and 13 higher for peptides in positive mode compared to the triple-tube interface. This first direct comparison of the three most common interfaces exhibits a strong improvement in sensitivity to the same extent for both nanoflow interfaces, where sheath liquid interfaces offer full flexibility in method development. |
| Databáze: | OpenAIRE |
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