Characterization and vaccination of two novel Schistosoma japonicum genes screened from a cercaria cDNA library
| Autor: | Xi-ya Wang, Shu-ju Zhang, Ying-ding Xia, Yunfei Zhou, Shi-ping Wang, Qi-mei Feng, Xue-qin Liu, Shao-hua Zeng, Dong-mei Gao, Zhi Tian |
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| Rok vydání: | 2011 |
| Předmět: |
Antibodies
Helminth Helminth genetics Enzyme-Linked Immunosorbent Assay Schistosoma japonicum law.invention Mice Plasmid Antigen law parasitic diseases Vaccines DNA Animals Humans Cercaria Parasite Egg Count Gene Library Vaccines Synthetic General Veterinary biology cDNA library Immunogenicity Uterus Vaccination General Medicine biology.organism_classification Virology Intestines Infectious Diseases Insect Science Antigens Helminth Schistosomiasis japonica Recombinant DNA biology.protein Parasitology Female Antibody Plasmids |
| Zdroj: | Parasitology research. 110(1) |
| ISSN: | 1432-1955 |
| Popis: | Two novel genes, SJCWL05 and SJCWL06, were harvested from screening of Schistosoma japonicum (S. japonicum) cercaria cDNA library by using pig sera vaccinated (VPS) with S. japonicum immature egg ws-vaccine (S. japonicum iEw). Prokaryotic recombinant plasmids pGEX-4T-1/SJCWL05 and pGEX-4T-1/SJCWL06 were constructed to analyze their immunogenicity, which was confirmed by SDS-PAGE and Western blotting. Two eukaryotic recombinant plasmids, pcDNA3/SJCWL05 and pcDNA3/SJCWL06, were constructed, and their ability to protect mice against challenge of S. japonicum was evaluated. All mice vaccinated with pcDNA3/SJCWL05 or pcDNA3/SJCWL06 developed ELISA-specific anti-S. japonicum SIEA (S. japonicum soluble immature egg antigens) antibody. Immunoprotection experiments showed that worms and liver eggs reduced 34.64% and 39.14% in the pcDNA3/SJCWL05 group and those reduced 27.17% and 27.95% in the pcDNA3/SJCWL06 group, respectively. The reduction rates of intestine and uterine eggs in female worms of both groups reached 39.45% and 38.5% as well as 30.02% and 28.7%, respectively. Results of our study suggest that novel genes, SJCWL05 and SJCWL06, are potential vaccine candidates against schistosomiasis japonica. |
| Databáze: | OpenAIRE |
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