Mitotic Transcription Repression in Vivo in the Absence of Nucleosomal Chromatin Condensation
Autor: | Charlotte A. Spencer, Michael J. Kruhlak, Xuejun Sun, Heather L. Jenkins, David P. Bazett-Jones |
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Rok vydání: | 2000 |
Předmět: |
chromosomes
Transcription Genetic RNA polymerase II Herpesvirus 1 Human Biology Virus Replication 03 medical and health sciences CDC2 Protein Kinase Aspartic Acid Endopeptidases Humans Gene Silencing Interphase Mitosis Transcription factor RNA polymerase II holoenzyme In Situ Hybridization Fluorescent Dyes 030304 developmental biology mitosis 0303 health sciences General transcription factor 030302 biochemistry & molecular biology Cell Biology Molecular biology Chromatin Cell Compartmentation Nucleosomes Bromodeoxyuridine Premature chromosome condensation DNA Viral biology.protein Original Article Transcription factor II E transcription HeLa Cells Transcription Factors |
Zdroj: | The Journal of Cell Biology |
ISSN: | 1540-8140 0021-9525 |
Popis: | All nuclear RNA synthesis is repressed during the mitotic phase of the cell cycle. In addition, RNA polymerase II (RNAP II), nascent RNA and many transcription factors disengage from DNA during mitosis. It has been proposed that mitotic transcription repression and disengagement of factors are due to either mitotic chromatin condensation or biochemical modifications to the transcription machinery. In this study, we investigate the requirement for chromatin condensation in establishing mitotic transcription repression and factor loss, by analyzing transcription and RNAP II localization in mitotic cells infected with herpes simplex virus type 1. We find that virus-infected cells enter mitosis and that mitotic viral DNA is maintained in a nucleosome-free and noncondensed state. Our data show that RNAP II transcription is repressed on cellular genes that are condensed into mitotic chromosomes and on viral genes that remain nucleosome free and noncondensed. Although RNAP II may interact indirectly with viral DNA during mitosis, it remains transcriptionally unengaged. This study demonstrates that mitotic repression of transcription and loss of transcription factors from mitotic DNA can occur independently of nucleosomal chromatin condensation. |
Databáze: | OpenAIRE |
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