Interleukin 1 alpha (IL-1α) restricts Brucella abortus 544 survival through promoting lysosomal-mediated killing and NO production in macrophages
Autor: | Man Hee Rhee, Chang Keun Kang, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Hu Jang Lee, Suk Kim, Tran Xuan Ngoc Huy, Lauren Togonon Arayan, Son Hai Vu, Wongi Min |
---|---|
Rok vydání: | 2019 |
Předmět: |
Interleukin-1beta
Brucella abortus Biology Nitric Oxide Real-Time Polymerase Chain Reaction Microbiology 03 medical and health sciences Mice Interleukin-1alpha CEBPA CEBPB Animals Transcription factor 030304 developmental biology 0303 health sciences Innate immune system Microbial Viability General Veterinary 030306 microbiology Macrophages Interleukin Receptors Interleukin-1 General Medicine Immunity Innate RAW 264.7 Cells Myeloid Differentiation Factor 88 Tumor necrosis factor alpha RNA Interference Signal transduction Signal Transduction Transcription Factors |
Zdroj: | Veterinary microbiology. 232 |
ISSN: | 1873-2542 |
Popis: | The interleukin-1 (IL-1) family of cytokines, particularly IL-1α and IL-1β, are potent regulators of innate immunity that play key roles in host defense against infection, hence we evaluated the role of these cytokines in the control of brucellosis within RAW 264.7 cells. Marked expression and secretion of IL-1α and IL-1β were observed during Brucella infection in macrophages. Blocking of IL-1α and IL-1β reduced induction of IL-10, IL-1β and TNF, and IL-6 and TNF, respectively. However, interference of IL-1α and not IL-1β signaling notably augmented susceptibility of macrophages to Brucella infection which indicates that IL-1α is required for a downstream signaling cascade of innate immunity for efficient clearance of Brucella. This protection requires binding to interleukin-1 receptor (IL-1R) mediated by myeloid differentiation factor 88 (MyD88) signaling and associated with increased lysosomal-mediated killing and nitric oxide (NO) production. Expression of pro-inflammatory cytokines was observed to be mediated via NF-κB-p50, HIF-1α and CEBPA, but negatively controlled by CEBPB while transcription of some important phagolysosomal genes was regulated via CEBPA and c-Jun which indicates the important role of these transcription factors in the control of Brucella infection in macrophages via IL-1α signaling pathway. |
Databáze: | OpenAIRE |
Externí odkaz: |