Discovery of Culex pipiens associated tunisia virus: a new ssRNA(+) virus representing a new insect associated virus family
Autor: | Philippe Gayral, Diane Bigot, Célestine M. Atyame, Mylène Weill, Elisabeth A. Herniou, Fabienne Justy |
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Přispěvatelé: | Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Culex pipiens mosquitoes Plasmodium RNA virus viruses 030106 microbiology Microbiology Genome Deep sequencing Virus Virgaviridae 03 medical and health sciences Virology Plant virus Culex pipiens ORFS Genetics virus discovery biology biology.organism_classification 030104 developmental biology [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology Research Article CpATV |
Zdroj: | Virus Evolution Virus Evolution, 2018, 4 (1), pp.vex040. ⟨10.1093/ve/vex040⟩ Virus Evolution, Oxford University Press, 2018, 4 (1), pp.vex040. ⟨10.1093/ve/vex040⟩ |
ISSN: | 2057-1577 |
Popis: | International audience; In the global context of arboviral emergence, deep sequencing unlocks the discovery of new mosquito-borne viruses. Mosquitoes of the species Culex pipiens, C. torrentium, and C. hortensis were sampled from 22 locations worldwide for transcriptomic analyses. A virus discovery pipeline was used to analyze the dataset of 0.7 billion reads comprising 22 individual transcriptomes. Two closely related 6.8 kb viral genomes were identified in C. pipiens and named as Culex pipiens associated tunisia virus (CpATV) strains Ayed and Jedaida. The CpATV genome contained four ORFs. ORF1 possessed helicase and RNA-dependent RNA polymerase (RdRp) domains related to new viral sequences recently found mainly in dipterans. ORF2 and 4 contained a capsid protein domain showing strong homology with Virgaviridae plant viruses. ORF3 displayed similarities with eukaryotic Rhoptry domain and a merozoite surface protein (MSP7) domain only found in mosquito-transmitted Plasmodium, suggesting possible interactions between CpATV and vertebrate cells. Estimation of a strong purifying selection exerted on each ORFs and the presence of a polymorphism maintained in the coding region of ORF3 suggested that both CpATV sequences are genuine functional viruses. CpATV is part of an entirely new and highly diversified group of viruses recently found in insects, and that bears the genomic hallmarks of a new viral family. |
Databáze: | OpenAIRE |
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