Methylation status of blood leukocyte DNA and risk of gastric cancer in a high-risk Chinese population
| Autor: | Ji-You Li, Isao Oze, Jun-Ling Ma, Wei-Cheng You, Kai-Feng Pan, Wei-Dong Liu, Keitaro Matsuo, Yang Zhang, Yasuhito Yuasa, Lian Zhang, Hui-juan Su, Lin Shen |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Epidemiology Atrophic gastritis Population Biology Gastroenterology Polymerase Chain Reaction Cohort Studies Asian People Insulin-Like Growth Factor II Stomach Neoplasms Internal medicine medicine Biomarkers Tumor Leukocytes Humans education Aged education.field_of_study Tumor Suppressor Proteins Cancer Intestinal metaplasia Membrane Proteins Methylation DNA Methylation Middle Aged medicine.disease Oncology Dysplasia Immunology DNA methylation Disease Progression Female Precancerous Conditions Cohort study |
| Zdroj: | Cancer epidemiology, biomarkersprevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 23(10) |
| ISSN: | 1538-7755 |
| Popis: | Background: To evaluate the relationship between methylation status of blood leukocyte DNA and risk of gastric cancer, a population-based study was conducted in Linqu County. Methods: Methylation levels of IGFII and N33 were determined by quantitative methylation-specific PCR. The temporal trend of methylation levels during gastric cancer development was investigated in 133 gastric cancer cases from two cohorts with pre– and/or post–gastric cancer samples. As the references of pre-GCs, 204 intestinal metaplasia (IM) or dysplasia (DYS) subjects who did not progress to gastric cancer during the follow-up period were selected. Meanwhile, 285 subjects with superficial gastritis/chronic atrophic gastritis (SG/CAG) were also selected as controls. Results: IGFII median methylation level was significantly higher in gastric cancer cases than those with SG/CAG (61.47% vs. 49.73%; P < 0.001). IGFII and N33 methylation levels were elevated at least 5 years ahead of clinical gastric cancer diagnosis comparing with SG/CAG (63.38% vs. 49.73% for IGFII, 9.12% vs. 5.70% for N33; all P < 0.001). Furthermore, the frequency of hypermethylated IGFII was markedly increased in IM or DYS subjects who progressed to gastric cancer in contrast to those who remained with IM and DYS, and adjusted ORs were 12.52 [95% confidence interval (CI), 3.81–41.15] for IM and 10.12 (95% CI, 2.68–38.22) for DYS. Similar results were also found for N33 in subjects with IM (OR, 3.77; 95% CI, 1.20–11.86). Conclusions: Our findings suggested that hypermethylated IGFII and N33 in blood leukocyte DNA were associated with risk of gastric cancer in a Chinese population. Impact: IGFII and N33 methylation status may be related to gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev; 23(10); 2019–26. ©2014 AACR. |
| Databáze: | OpenAIRE |
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