Novel ultrastructural findings on cardiac mitochondria of huddling Brandt's voles in mild cold environment
| Autor: | Lai-xiang Xu, Jinhui Xu, Jun-Jie Mou, Huiliang Xue, Zhe Wang, Xiao-Tong Kong, Ming Wu, Jian-wen Zou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
030110 physiology 0301 basic medicine medicine.medical_specialty Mitochondrial fission factor Physiology Apoptosis PINK1 Mitochondrion Biochemistry Mitochondria Heart 03 medical and health sciences Lasiopodomys brandtii Internal medicine Autophagy medicine Animals Social Behavior Molecular Biology bcl-2-Associated X Protein Cell Nucleus Behavior Animal biology Arvicolinae Caspase 3 Chemistry Body Weight biology.organism_classification Adaptation Physiological Mitochondria Cold Temperature 030104 developmental biology Endocrinology DNA fragmentation Phosphorylation Female Mitochondrial fission |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 249:110766 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2020.110766 |
| Popis: | Reduced ambient temperature has a damaging effect on mammalian myocardium. Huddling as a cooperative behavior has evolved in social mammals as a strategy to maximize adaptation to environmental cooling. Here, we studied the effects of huddling behavior on mitochondrial morphology, number, and function in the myocardia of Brandt's voles (Lasiopodomys brandtii) under cool environmental temperatures (15 °C). Results showed (1) mitochondrial swelling and cristae disruption in the cool huddling group (CH) and cool separated group (CS). Compared to the control group (CON, 22 °C), damaged mitochondria in the cool huddling and separated groups reached >90%; however, total number of mitochondria in the CH group was similar to that in the CON group. (2) ATP synthase activity was lowest in the CS group, whereas citrate synthetase activity was maintained among the three treatment groups. (3) Bax/bcl2 protein expression in the CH and CS groups was higher than that in the CON group, whereas DNA fragmentation, nuclear number, and caspase3 activity showed no significant differences among the three groups. (4) The protein expression levels of dynamin-related protein1 and mitochondrial fission factor were highest in the CH group. (5) Both protein expression of PINK1 and phosphorylation ratio of Parkin showed the pattern CS > CH > CON. (6) Total number of mitochondria was higher in males than in females. In general, the increased mitochondrial fission level observed in huddling voles partially counteracted the decrease in myocardial mitochondria caused by the increase in autophagy. |
| Databáze: | OpenAIRE |
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