Serum antibodies to phosphatidylcholine in MS
| Autor: | Howard L. Weiner, Esther Escudero, Maria Cruz Sádaba, Francisco J. Quintana, Veit Rothhammer, Stephen L. Hauser, Roberto Alvarez-Lafuente, Rohit Bakshi, Cristina Sebal, Pia Kivisäkk, Guillermo Izquierdo, Sergio E. Baranzini, Jorge R. Oksenberg, Maria Isabel Garcia Sanchez, Ursula Munoz |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Neurodegenerative Gastroenterology Immunoglobulin G Cohort Studies chemistry.chemical_compound 0302 clinical medicine 80 and over Aged 80 and over music.instrument Clinically isolated syndrome biology Middle Aged Neurology Phosphatidylcholines Biomarker (medicine) Female Antibody Adult medicine.medical_specialty Multiple Sclerosis Lactosylceramides Autoimmune Disease Article 03 medical and health sciences Lactosylceramide Young Adult Internal medicine Phosphatidylcholine medicine Humans In patient music Autoantibodies Aged business.industry Prevention Neurosciences Brain Disorders 030104 developmental biology chemistry Immunoglobulin M biology.protein Neurology (clinical) business 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Neurology(R) neuroimmunology & neuroinflammation, vol 7, iss 4 Neurology® Neuroimmunology & Neuroinflammation |
| Popis: | Author(s): Sadaba, Maria Cruz; Rothhammer, Veit; Munoz, Ursula; Sebal, Cristina; Escudero, Esther; Kivisakk, Pia; Garcia Sanchez, Maria Isabel; Izquierdo, Guillermo; Hauser, Stephen L; Baranzini, Sergio E; Oksenberg, Jorge R; Alvarez-Lafuente, Roberto; Bakshi, Rohit; Weiner, Howard L; Quintana, Francisco J | Abstract: ObjectiveTo evaluate the value of serum immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies reactive with phosphatidylcholine (PC) and lactosylceramide (LC) as biomarkers in MS.MethodsWe developed an ultrasensitive ELISA technique to analyze serum IgG and IgM antibodies to LC and PC, which we used to analyze samples from 362 patients with MS, 10 patients with non-MS myelin diseases (Non-MSMYDs), 11 patients with nonmyelin neurologic diseases (Non-MYNDs), and 80 controls. MS serum samples included clinically isolated syndrome (CIS, n = 17), relapsing-remitting MS (RRMS, n = 62), secondary progressive MS (SPMS, n = 50), primary progressive MS (PPMS, n = 37), and benign MS (BENMS, n = 36).ResultsWe detected higher levels of serum IgM antibodies to PC (IgM-PC) in MS than control samples; patients with CIS and RRMS showed higher IgM-PC levels than patients with SPMS, PPMS, and BENMS and controls. MS and control samples did not differ in serum levels of IgM antibodies reactive with LC, nor in IgG antibodies reactive with LC or PC.ConclusionsSerum IgM-PC antibodies are elevated in patients with MS, particularly during the CIS and RRMS phases of the disease. Thus, serum IgM-PC is a candidate biomarker for early inflammatory stages of MS.Classification of evidenceThis study provides Class III evidence that serum antibodies to PC are elevated in patients with MS. The study is rated Class III because of the case control design and the risk of spectrum bias: antibody levels in patients with MS were compared with healthy controls. |
| Databáze: | OpenAIRE |
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