Online monitoring of biofilm growth and activity using a combined multi-channel impedimetric and amperometric sensor
Autor: | Leonardo Pires, Thomas Schwartz, Tanja Kleintschek, Kai Sachsenheimer, Ansgar Waldbaur, Bastian E. Rapp |
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Rok vydání: | 2013 |
Předmět: |
Biocide
Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques medicine.disease_cause chemistry.chemical_compound Electrochemistry medicine Electrodes Chemistry Pseudomonas aeruginosa Biofilm General Medicine biochemical phenomena metabolism and nutrition Contamination Amperometry Dielectric spectroscopy Biofilms Dielectric Spectroscopy Sodium azide Biosensor Disinfectants Biotechnology |
Zdroj: | Biosensors and Bioelectronics. 47:157-163 |
ISSN: | 0956-5663 |
Popis: | Biofilms are ubiquitous in water interfaces and therefore influence our daily lives in an ambivalent manner. In medicine, infections can be attributed to biofilm formation. In technical systems, biofilms are causative agents for biocorrosion, contamination, and clogging processes and are responsible for shear force modification in marine systems. To control and manipulate biofilm formation advanced technologies are needed. This paper reports on a novel real-time biofilm monitoring system using custom-made electronics. The system is able to monitor four electrochemical impedance spectroscopy (EIS) electrodes and three amperometric sensors in two microfluidic channels assessing biofilm growth and activity in parallel using Pseudomonas aeruginosa as a model system. The biofilm was characterized during its seeding and growth stages as well as during different injection intervals of a biocide (sodium azide) which allowed monitoring biofilm destabilization and deactivation effects in real time. The results obtained were confirmed by fluorescence microscopy after live/dead cell staining of the bacteria in the measured biofilm. |
Databáze: | OpenAIRE |
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