Vaccination of yearling horses against poly-N-acetyl glucosamine fails to protect against infection with Streptococcus equi subspecies equi

Autor: Gerald B. Pier, Colette Cywes-Bentley, Angela I. Bordin, Susanne M Kahn, S Garrett Wehmeyer, Jocelyne M. Bray, Noah D. Cohen
Rok vydání: 2020
Předmět:
Male
Physiology
Biochemistry
0403 veterinary science
Streptococcus equi subspecies equi
Medical Conditions
Immune Physiology
Medicine and Health Sciences
Public and Occupational Health
Enzyme-Linked Immunoassays
Booster Doses
Strangles
Mammals
Vaccines
0303 health sciences
Immune System Proteins
Multidisciplinary
biology
Vaccination
Eukaryota
04 agricultural and veterinary sciences
Vaccination and Immunization
Antibodies
Bacterial
Infectious Diseases
Vertebrates
Medicine
Female
Antibody
Research Article
Infectious Disease Control
040301 veterinary sciences
Science
Equines
Immunology
Research and Analysis Methods
Injections
Intramuscular
Antibodies
Acetylglucosamine
Microbiology
03 medical and health sciences
Antigen
Streptococcal Infections
Animals
Streptococcus equi
Horses
Antigens
Immunoassays
Secretion
030304 developmental biology
Organisms
Biology and Life Sciences
Proteins
Immunization
Amniotes
Immunologic Techniques
biology.protein
Horse Diseases
Nasal administration
Preventive Medicine
Physiological Processes
Zoology
Ex vivo
Zdroj: PLoS ONE, Vol 15, Iss 10, p e0240479 (2020)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0240479
Popis: Strangles is a common disease of horses with worldwide distribution caused by the bacterium Streptococcus equi subspecies equi (SEE). Although vaccines against strangles are available commercially, these products have limitations in safety and efficacy. The microbial surface antigen β 1→6 poly-N-acetylglucosamine (PNAG) is expressed by SEE. Here we show that intramuscular (IM) injection alone or a combination of IM plus intranasal (IN) immunization generated antibodies to PNAG that functioned to deposit complement and mediate opsonophagocytic killing of SEE ex vivo. However, immunization strategies targeting PNAG either by either IM only injection or a combination of IM and IN immunizations failed to protect yearling horses against infection following contact with infected horses in an experimental setting. We speculate that a protective vaccine against strangles will require additional components, such as those targeting SEE enzymes that degrade or inactivate equine IgG.
Databáze: OpenAIRE
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