Lipidomics of hepatic lipogenesis inhibition by omega 3 fatty acids
| Autor: | Antonin Lamaziere, Pedro Bausero, Francesco Visioli, Ullah Barbe, Claude Wolf |
|---|---|
| Přispěvatelé: | Université Pierre et Marie Curie - Paris 6 (UPMC), Inflammation intestinale, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2013 |
| Předmět: |
Fatty Acid Desaturases
Male Spectrometry Mass Electrospray Ionization Clinical Biochemistry Phospholipid Linoleoyl-CoA Desaturase chemistry.chemical_compound Delta-5 Fatty Acid Desaturase Fatty Acids Omega-3 Lipidomics Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Rats Wistar Phospholipids chemistry.chemical_classification Lipogenesis Substrate (chemistry) Fatty acid Cell Biology Fish oil Delta-6-desaturase Rats Enzyme Liver chemistry Biochemistry lipids (amino acids peptides and proteins) Omega 3 fatty acids Stearoyl-CoA Desaturase |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids Prostaglandins, Leukotrienes and Essential Fatty Acids, 2013, 88 (2), pp.149-154. ⟨10.1016/j.plefa.2012.12.001⟩ |
| ISSN: | 0952-3278 |
| DOI: | 10.1016/j.plefa.2012.12.001 |
| Popis: | International audience; We assessed - by a lipidomic approach - the differential incorporation of EPA and DHA into hepatic lipids, after prolonged feeding of rats with fish oil. We also evaluated their effect on lipogenesis and its related enzymes. Rats were administered 100 mg/kg/d fish oil, by oral gavage, for 30 days. The fatty acid profile of total liver lipids was determined by gas-liquid chromatography coupled to mass spectrometry. Individual phospholipid classes and their molecular species were quantified by ESI-MS/MS. Omega 3 fatty acids readily incorporated into hepatic phospholipids, decreased stearoyl-CoA desaturase 16, stearoyl-CoA desaturase, delta 6 desaturase, and delta 5 desaturase activities (calculated as product/substrate ratio) and decreased the ``lipogenesis index'', i.e., the proportion of fatty acids endogenously synthesized in the liver and not provided with the diet. Our results show that long-chain omega 3 fatty acids selectively incorporate into hepatic phospholipids, inhibit de novo lipogenesis and change the hepatic fatty acid profile via reduced desaturases' activity in the non-steatotic liver. In addition to corroborating advice to consume adequate amounts of omega 3 fatty acids for overall health, these data contribute mechanistic insights to the clinical observations that provision of omega 3 fatty acids decreases hepatic fat and ameliorates NAFLD prognosis. |
| Databáze: | OpenAIRE |
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