Genome-Wide Analysis and Expression Profiling of the Phospholipase D Gene Family in Solanum tuberosum
| Autor: | Qin Chen, Yue Chen, Mancang Zhang, Dongdong Wang, Chenghui Yang, Chao Zhang, Yanru Bao, Long Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine abiotic stress QH301-705.5 Biology 01 natural sciences Genome General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Gene expression Gene family Biology (General) Gene Genetics General Immunology and Microbiology Phospholipase D Abiotic stress fungi food and beverages Promoter Gene expression profiling 030104 developmental biology gene expression potato PLD General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Biology Volume 10 Issue 8 Biology, Vol 10, Iss 741, p 741 (2021) |
| ISSN: | 2079-7737 |
| DOI: | 10.3390/biology10080741 |
| Popis: | Simple Summary Phospholipase D is the most important kind of phospholipase in plants, which can specifically catalyze the hydrolysis of phosphodiester bonds at the end of phospholipid molecules to produce phospholipid acid and a free group. Phospholipase D is not only involved in maintaining the structural stability of plant cell membrane and the dynamic balance of lipid composition but also involved in a variety of physiological processes, such as plant growth and development and stress response, including stomatal closure, root elongation, cell senescence, high salt stress, cold stress, and drought stress. It plays a critical role in plant growth and development, as well as in hormone and stress responses. In this study, the basic information on phospholipase D gene family was comprehensively introduced, and the physical and chemical properties, systematic evolution, gene structure, conservative motif, chromosome location, gene replication, cis-acting element prediction, GO annotation, interspecific collinearity, and quantitative analysis of potato phospholipase D gene family were analyzed to gain a better understanding of potato phospholipase D gene family. Through the analysis of potato phospholipase D gene family, our results lay the foundation for further understanding of the function of phospholipase gene family in higher plants. Abstract Phospholipase D (PLD) is the most important phospholipid hydrolase in plants, which can hydrolyze phospholipids into phosphatidic acid (PA) and choline. When plants encounter low temperature, drought and high salt stress, phospholipase D and its products play an important role in regulating plant growth and development and coping with stress. In this study, 16 members of StPLD gene family were identified in potato genome, which were distributed in α, β, δ, and ζ subfamilies, and their expression patterns under salt, high temperature, drought, and ABA stress were detected by qRT-PCR method. Gene expression analysis showed that the expression of StPLD genes in potato was upregulated and downregulated to varying degrees under the four stresses, indicating that the PLD gene family is involved in the interaction of potato plant hormones and abiotic stress signals. Chromosome distribution showed that StPLD gene was unevenly distributed on 8 chromosomes, and only one pair of tandem repeat genes was found. All StPLD promoters contain hormone and stress-related cis-regulatory elements to respond to different stresses. Structural analysis showed that StPLD genes in the same subgroup had a similar exon–intron structure. Our study provides a valuable reference for further research of the function and structure of PLD gene. |
| Databáze: | OpenAIRE |
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