Fluorine-19 MRI at 21.1 T: enhanced spin–lattice relaxation of perfluoro-15-crown-5-ether and sensitivity as demonstrated in ex vivo murine neuroinflammation
| Autor: | Paula Ramos Delgado, Sonia Waiczies, João S. Periquito, Ludger Starke, Thoralf Niendorf, Christian Prinz, Andreas Pohlmann, Jens T. Rosenberg, Jason M. Millward, Helmar Waiczies, Andre Kuehne |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Encephalomyelitis
Autoimmune Experimental Radio Waves Biophysics chemistry.chemical_element Contrast Media Signal-To-Noise Ratio Sensitivity and Specificity 030218 nuclear medicine & medical imaging 03 medical and health sciences Mice 0302 clinical medicine Nuclear magnetic resonance In vivo Crown Ethers medicine Animals Radiology Nuclear Medicine and imaging Neuroinflammation Inflammation Experimental autoimmune encephalomyelitis Radiological and Ultrasound Technology Chemistry Fluorine-19 magnetic resonance imaging Spin–lattice relaxation Brain Reproducibility of Results Fluorine Perfluoro-15-crown-5-ether medicine.disease Signal-to-noise ratio (imaging) Magnetic fields Calibration Nanoparticles Female Spin Labels Lymph Nodes Ex vivo Spleen Research Article |
| Zdroj: | Magma (New York, N.y.) Magnetic Resonance Materials in Physics, Biology and Medicine |
| ISSN: | 1352-8661 0968-5243 |
| Popis: | Objective Fluorine MR would benefit greatly from enhancements in signal-to-noise ratio (SNR). This study examines the sensitivity gain of 19F MR that can be practically achieved when moving from 9.4 to 21.1 T. Materials and methods We studied perfluoro-15-crown-5-ether (PFCE) at both field strengths (B0), as a pure compound, in the form of nanoparticles (NP) as employed to study inflammation in vivo, as well as in inflamed tissue. Brains, lymph nodes (LNs) and spleens were obtained from mice with experimental autoimmune encephalomyelitis (EAE) that had been administered PFCE NPs. All samples were measured at both B0 with 2D-RARE and 2D-FLASH using 19F volume radiofrequency resonators together. T1 and T2 of PFCE were measured at both B0 strengths. Results Compared to 9.4 T, an SNR gain of > 3 was observed for pure PFCE and > 2 for PFCE NPs at 21.1 T using 2D-FLASH. A dependency of 19F T1 and T2 relaxation on B0 was demonstrated. High spatially resolved 19F MRI of EAE brains and LNs at 21.1 T revealed signals not seen at 9.4 T. Discussion Enhanced SNR and T1 shortening indicate the potential benefit of in vivo 19F MR at higher B0 to study inflammatory processes with greater detail. Electronic supplementary material The online version of this article (10.1007/s10334-018-0710-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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