Developmental toxicity and induction of vitellogenin in embryo-larval stages of zebrafish ( Danio rerio ) exposed to methyl Paraben
| Autor: | S. Barathi, Winkins Santosh, Vrinda Yatin Dambal, Christy Lite, Karthikeyan Ponnien Selvan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Embryo Nonmammalian animal structures Health Toxicology and Mutagenesis Danio Developmental toxicity Parabens Endocrine Disruptors 010501 environmental sciences 01 natural sciences Lethal Dose 50 Blood cell Vitellogenins 03 medical and health sciences Vitellogenin chemistry.chemical_compound Internal medicine medicine Animals Zebrafish 0105 earth and related environmental sciences Dose-Response Relationship Drug biology Methylparaben Hatching Reproduction fungi Public Health Environmental and Occupational Health Embryo General Medicine biology.organism_classification Pollution Up-Regulation 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Larva embryonic structures biology.protein Water Pollutants Chemical |
| Zdroj: | Ecotoxicology and Environmental Safety. 141:113-118 |
| ISSN: | 0147-6513 |
| DOI: | 10.1016/j.ecoenv.2017.02.048 |
| Popis: | MethylParaben (MP), a methyl ester of p-hydroxybenzoic acid, is used as an anti-microbial preservative in foods, drugs and cosmetics for decades. It enters the aquatic environment, and can have toxic effects on aquatic organisms. Little is known on the developmental toxicity of MP exposure to zebrafish during early life stages. In this study, the developmental effects of MP were evaluated in embryo-larval zebrafish (at concentrations ranging from 100μM, 200μM, 400µM, 800μM and 1000μM for 96h post fertilization (hpf). The survival, hatching, heart beat rate and developmental abnormalities were observed in the embryos exposed to MP. MP exposure resulted in decreased heart rate and hatching rate. Defects including pericardial edema blood cell accumulation and bent spine were observed in all the treated concentration, except at 100μM. With increasing concentrations, the frequency of these defects increased. The 96 hpf LC50 of MP was calculated to be 428μM (0.065mg/L). Furthermore, RT-PCR result showed that in larval zebrafish exposed to 100μM (0.015mg/L) of MP till 96 hpf, expression of vitellogenin I (Vtg -I) was significantly upregulated compared to the control group. This data suggest that even though lower concentrations of MP do not cause phenotypic malformations, it leads to dysregulated expression of estrogenic biomarker gene Vtg-I. |
| Databáze: | OpenAIRE |
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