Insulin degrading enzyme is expressed in the human cerebrovascular endothelium and in cultured human cerebrovascular endothelial cells
| Autor: | Hamant S. Thatte, Patricia B. Eisenhauer, M. David Ullman, Kelly J. Conn, John A. Lynch, John M. Wells, Ann C. McKee, Richard E. Fine, Wenwu Gao |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.diagnostic_test
biology Endothelium Amyloid beta Microcirculation General Neuroscience Insulin medicine.medical_treatment Immunofluorescence Insulysin Molecular biology Gene Expression Regulation Enzymologic Substantia Nigra Endothelial stem cell medicine.anatomical_structure Biochemistry Cerebrovascular Circulation medicine biology.protein Insulin-degrading enzyme Humans Endothelium Vascular Cells Cultured Laser capture microdissection |
| Zdroj: | Neuroscience Letters. 371:6-11 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2004.07.034 |
| Popis: | Insulin degrading enzyme (IDE) is found in the cytosol, peroxisomes and plasma membrane of many cells. Although it preferentially cleaves insulin it can also cleave many other small proteins with diverse sequences including the monomeric form of the amyloid beta peptide (A beta). In the brain, IDE has been reported to be expressed predominantly in neurons. In this study, IDE expression was detected in cultured human cerebrovascular endothelial cells. Using laser capture microdissection followed by PCR analysis, it was found that IDE mRNA is expressed in human brain blood vessels. Using immunofluorescence and multiphoton microscopy IDE was localized to the endothelium of the cerebrovascular blood vessels in human. |
| Databáze: | OpenAIRE |
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