A human pericardium biopolymeric scaffold for autologous heart valve tissue engineering: cellular and extracellular matrix structure and biomechanical properties in comparison with a normal aortic heart valve

Autor:	Frantisek Straka, David Schornik, Zdenek Svindrych, Jaroslav Masin, Jelena Skibová, Jiri Machac, Hynek Chlup, Lukas Horny, Jan Pirk, Zuzana Burdikova, Elena Filova, Tomas Mirejovsky, Lucie Bacakova, Matej Daniel
Rok vydání:	2018
Předmět:	0301 basic medicine Lamina Pathology medicine.medical_specialty Scaffold Biomedical Engineering Biophysics Bioengineering Biomaterials Glycosaminoglycan Extracellular matrix 03 medical and health sciences Biopolymers Tensile Strength Materials Testing medicine Humans Pericardium Heart valve Aorta Mechanical Phenomena Tissue Engineering Tissue Scaffolds biology Chemistry Mesenchymal stem cell Heart Valves Biomechanical Phenomena Extracellular Matrix 030104 developmental biology medicine.anatomical_structure biology.protein Elastin
Zdroj:	Journal of Biomaterials Science, Polymer Edition. 29:599-634
ISSN:	1568-5624 0920-5063
Popis:	The objective of our study was to compare the cellular and extracellular matrix (ECM) structure and the biomechanical properties of human pericardium (HP) with the normal human aortic heart valve (NAV). HP tissues (from 12 patients) and NAV samples (from 5 patients) were harvested during heart surgery. The main cells in HP were pericardial interstitial cells, which are fibroblast-like cells of mesenchymal origin similar to the valvular interstitial cells in NAV tissue. The ECM of HP had a statistically significantly (p 0.001) higher collagen I content, a lower collagen III and elastin content, and a similar glycosaminoglycans (GAGs) content, in comparison with the NAV, as measured by ECM integrated density. However, the relative thickness of the main load-bearing structures of the two tissues, the dense part of fibrous HP (49 ± 2%) and the lamina fibrosa of NAV (47 ± 4%), was similar. In both tissues, the secant elastic modulus (Es) was significantly lower in the transversal direction (p 0.05) than in the longitudinal direction. This proved that both tissues were anisotropic. No statistically significant differences in UTS (ultimate tensile strength) values and in calculated bending stiffness values in the longitudinal or transversal direction were found between HP and NAV. Our study confirms that HP has an advantageous ECM biopolymeric structure and has the biomechanical properties required for a tissue from which an autologous heart valve replacement may be constructed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a875c444d260f7e5c6bb2793b695cc82 https://doi.org/10.1080/09205063.2018.1429732 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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