Enzyme-assisted vitrectomy in enucleated pig eyes: a comparison of hyaluronidase, chondroitinase, and plasmin
| Autor: | Flemming Staubach, P. Janknecht, Veronika Nober |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Time Factors Swine Plasmin medicine.medical_treatment Hyaluronoglucosaminidase Vitrectomy Eye Enucleation Cellular and Molecular Neuroscience Hyaluronidase Ophthalmology medicine Animals Fibrinolysin Incubation chemistry.chemical_classification Retina Retinal damage Sensory Systems Chondroitinases and Chondroitin Lyases Surgery Vitreous Body Enzyme medicine.anatomical_structure chemistry medicine.drug |
| Zdroj: | Current Eye Research. 29:261-268 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.1080/02713680490516747 |
| Popis: | Facilitation of vitrectomy by vitreolytic enzymes may be of great value in complicated or office-procedure vitreo-retinal surgery. In this study, we quantified and compared the effect of hyaluronidase, chondroitinase, and plasmin pre-incubation on vitrectomy rate and explored potential retinal damage.Freshly enucleated pigs eyes were incubated (1 or 3 hours) with an intravitreally injected enzyme or control solution. Enzyme doses were 100 and 1000 U for hyaluronidase, 1 and 2 U for chondroitinase, 3 and 30 U for plasmin. The eyes were weighed before and after 10 minutes of one-port vitrectomy, the difference representing the amount of removed vitreous. Light microscopy was used to assess potential damage to the retina.All enzymes significantly increased the amount of removed vitreous at all doses and incubation periods. The highest increase was found with hyaluronidase 1000 U, 3 hours, the lowest with chondroitinase 1 U, 1 hour. Damage occasionally occurred to the internal limiting membrane and very rarely to the nerve fiber layer. No damage at all was seen in the 100 and 1000 U hyaluronidase (1-hour incubation) groups.Hyaluronidase, chondroitinase, and plasmin are good candidates for enzyme-assisted vitrectomy. Although retinal structural damage was very rarely seen, safety concerns will have to be investigated further. |
| Databáze: | OpenAIRE |
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