Characterization of tissue and functional deficits in a clinically translational pig model of acute ischemic stroke
| Autor: | Madison M. Fagan, Hea Jin Park, Soo K. Shin, Kelly M. Scheulin, Erin E. Kaiser, Simon R. Platt, Elizabeth S. Waters, Holly A. Kinder, Julie Jeon, Xi Fang, Franklin D. West, Kylee J. Duberstein |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Internal capsule Neutrophils Swine Brain Ischemia Brain ischemia White matter 03 medical and health sciences 0302 clinical medicine Midline shift Internal medicine Fractional anisotropy Medicine Animals Lymphocytes Molecular Biology Stroke Gait Ischemic Stroke Intracerebral hemorrhage medicine.diagnostic_test business.industry Depression General Neuroscience Magnetic resonance imaging Infarction Middle Cerebral Artery medicine.disease Magnetic Resonance Imaging White Matter Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cardiology Neurology (clinical) business Gait Analysis Intracranial Hemorrhages 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain research. 1736 |
| ISSN: | 1872-6240 |
| Popis: | The acute stroke phase is a critical time frame used to evaluate stroke severity, therapeutic options, and prognosis while also serving as a major tool for the development of diagnostics. To further understand stroke pathophysiology and to enhance the development of treatments, our group developed a translational pig ischemic stroke model. In this study, the evolution of acute ischemic tissue damage, immune responses, and functional deficits were further characterized. Stroke was induced by middle cerebral artery occlusion in Landrace pigs. At 24 h post-stroke, magnetic resonance imaging revealed a decrease in ipsilateral diffusivity, an increase in hemispheric swelling resulting in notable midline shift, and intracerebral hemorrhage. Stroke negatively impacted white matter integrity with decreased fractional anisotropy values in the internal capsule. Like patients, pigs showed a reduction in circulating lymphocytes and a surge in neutrophils and band cells. Functional responses corresponded with structural changes through reductions in open field exploration and impairments in spatiotemporal gait parameters. Characterization of acute ischemic stroke in pigs provided important insights into tissue and functional-level assessments that could be used to identify potential biomarkers and improve preclinical testing of novel therapeutics. |
| Databáze: | OpenAIRE |
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